| 用~3H和地高辛配基标记TNF—α cDNA探针进行原位杂交的比较 |
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| 引用本文： | 刘天菊,司履生,杨居祥.用~3H和地高辛配基标记TNF—α cDNA探针进行原位杂交的比较[J].西安交通大学学报(医学版),1992(3). |
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| 作者姓名： | 刘天菊 司履生 杨居祥 |
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| 作者单位： | 西安医科大学免疫病理研究室，西安医科大学免疫病理研究室，西安医科大学免疫病理研究室 西安 710061，西安 710061，西安 710061 |
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| 摘 要： | 用~3H和地高辛配基标记肿瘤坏死因子-αcDNA探针以及原位杂文技术检测33例子宫颈癌和28例阴道尖锐湿疣活检标本中的TNF mRNA。结果表明,两种标记探针原位杂交结果基本一致,~3H标记探针敏感性稍高于地高辛配基标记探针。但放射性核素探针的固有缺点及非同位素探针的安全、快速、方便使非同位素探针有着日益广泛的应用前景。
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| 关 键 词： | ~3H标记探针 地高辛配基标记探针 原位杂交 |
THE COMPARISION OF IN SITU HYBRIDIZATION BY USING ~3H WITH DIGOXIGENIN LABELED TNF PROBE |
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| Liu Tianju,Si Lusheng,Yang Juxiang.THE COMPARISION OF IN SITU HYBRIDIZATION BY USING ~3H WITH DIGOXIGENIN LABELED TNF PROBE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1992(3). |
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| Authors: | Liu Tianju Si Lusheng Yang Juxiang |
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| Institution: | Immunopathology Insititute |
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| Abstract: | The TNA mRNA expression in 33 samples of cervical carcinoma and 28 samples of condyloma have been observed by using ~3H and Digoxigenin labeled TNF—α cDNA probes and in situ hybridization technique, we found that the results of in situ hybrdfzation with these two kinds of labeled probes were alike, the sensitivity with ~3H labeled probe was slightly higher than that with Digoxigenin labeled probe. Nevertheless, the nonisotope probe would be used more and more in future because of its safety, rapidity and convenience in work. |
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| Keywords: | ~3H labeled TNF probe Digoxigenin labeled TNF probe in situ hybrdization |
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