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SIMPLE METHOD OF PUNCHING AND RE-LOCATING TISSUES FOR MANUAL CONSTRUCTION OF TISSUE MICROARRAY |
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| Authors: | ZHENG Lin ZHANG Fan WANG Li YAN Zhao-wen JIANG Xu-cheng Pierre-tienne Bouchettel-Dieu de Paris France) |
| Affiliation: | ZHENG Lin(Department of Pathology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)；ZHANG Fan(Department of Pathology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)；WANG Li(Department of Pathology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)；YAN Zhao-wen(Department of Pathology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)；JIANG Xu-cheng(Department of Pathology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China)；Pierre-(E)tienne Bouchet(Service d'anatomie pathologique, H(o)tel-Dieu de Paris, France)； |
| Abstract: | A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder ( 1.2 - 1.8mm) were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray (TMA) block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization. |
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