Abstract: | For establishing a method of diagnosing morphine poisoning from pathological tissue slide and studying on morphine distribution, we used immunofluorescence technique to observe in situs whether morphine was present in tissues of rabbits with chronic administration of morphine hy-drochloride and concentration differences among poi-soned rabbit organs. Meantime, we also used HPLC to detect morphine concentration in same tissue samples from rabbit. By comparing and analysing the two methods, morphine immunofluorescence technique established in this experiment can be used as a convenient, fast, specific method for identifying morphine poisoning. |