用聚合酶链反应检测口腔癌组织中人乳头状瘤病毒的E_6转化基因

作者采用聚合酶链反应（ＰＣＲ），并对照核酸斑点杂交法对１０例口腔恶性肿瘤组织中ＨＰＶ１６Ｅ６转化基因进行了检测。这些肿瘤包括６例口腔粘膜鳞癌（ＳＣＣ），２例涎腺腺样囊性癌（ＡＣＣ），１例涎腺恶性多形性腺瘤（ＭＰＡ）和１例腭部软组织胚胎性横纹肌肉瘤（ＥＲ）。对照组为１０例非肿瘤组织，包括唇裂、腭裂粘膜组织标本各５例。结果表明两种方法检测结果基本一致，肿瘤组阳性６例（６／１０），包括４例ＳＳＣ，１例ＡＣＣ和１例ＥＲ（除外１例ＡＣＣ用核酸杂交法检测为弱阳性），其余为阴性；对照组用核酸杂交法检测４例，除１例唇裂标本为弱阳性以外，均为阴性（０／４）；用ＰＣＲ法检测１０例均为阴性结果（０／１０）。两组间ＨＰＶ１６Ｅ６转化基因的检出率有明显差异（Ｐ＜０．０５）。这一结果为开展口腔癌的ＨＰＶ病因学研究提供了参考资料。

DETECTION OF E_6 TRANSFORMATION GENE OF HUMAN PAPILLOMAVIRUS TYPE 16 IN ORAL CANCERS USING POLYMERASE CHAIN REACTIONS

The polymerase chain reaction (PCR) technique, compared .with dot-blot DNA hybridization, was used to detect E6 transformation gene of human papillomavirus type 16, in the tissues of 10 oral malignant tumors. The tumors were : 6 oral mucosal squamous cell carcinomas (SCC), 2 salivary glandular adenoid cystic carcinomas (ACC), 1 malignant pleomorphic adenoma (MPA), and 1 embryoid rhabdomyosarcoma (ER) in the soft tissue of the palate, 10 non-tumor cases includuig 5 cleft lips and 5 cleft palates were chosen for control. The results with the two techniques were basically. identical. 6 out of the 10 (6/10) tumors, which were 4 SCC, 1 ACC and 1 ER, were positive for the E_6 transformation.gene in the non-tumor control,none of the 4 (0/4) cases detected with dot-blot DNA hybridization showed a definite positive result,except for 1 cleft lip with a weakly positive result.All the 10 non-tumor cases detected with PCR showed negtive results (0/10). The difference of the positive rate between the two groups was significant (P<0. 05). The results provided data for the study on HPV virological oncogenesis of the oral Cancers.