小儿过敏性紫癜血清炎前因子的检测及其意义

目的　探讨炎前因子在过敏性紫癜免疫病理损伤中的作用。方法　采用双抗体夹心ELISA法和硝酸还原酶化学比色法分别检测过敏性紫癜 (SHP)患儿血清肿瘤坏死因子α(TNFα)、白细胞介素 6(IL 6)、白细胞介素 8(IL 8)和一氧化氮 (NO)的浓度。结果　SHP急性期血清 4种炎前因子水平明显高于正常对照组 (P <0 .0 5 )。肾型中活动性肾脏病和 (或 )肾功能不全组较其他组血清IL 6明显升高 (P <0 .0 1 )。恢复期TNF α、IL 8仍高于正常对照组 (P <0 .0 5 )。急性期 4种炎前因子间呈正相关关系。结论　血清 4种炎前因子水平与SHP的发生、发展有关。

Determination of serum proinflammatory factor ani its significance in children with scholein henoch purpura

Objective To evaluate the pathological effects of proinflammatory factor in children with scholein henoch purpura(SHP). Methods Double antibody sandwich enzyme linked immunosorbent assary and nitrate reductase were used to determine serum tumor necrotic factor α (TNF α),interleukin 6(IL 6),interleukin 8(IL 8) and nitric oxide (NO) in children with scholein henoch purprua.Results Level of TNFα,IL 6,IL 8 and NO in serum during acute stage was significant higher than that in normal( P < 0.05 ). There also was a significant positive correlation.It was observed that the TNF α and IL 8 level in the convalescent stage appeared significant higher than that in normal( P <0.05). In cases with SHP nephritis,the IL 6 level in patients with activiting kidney illness and (or) renal insufficiency was higher than that in the others( P <0.01).Conclusion The concentration of TNFα,IL 6,IL 8 and NO in serum might play a role in the pathogenesis and development in children with SHP.