酶联抗人IgG单克隆抗体与多克隆抗体测定PAIgG法的比较

作者应用辣根过氧化酶—抗人IgG单克隆抗体(HRP—McAb)和辣根过氧化酶—抗人IgG多克隆抗体(HRP—PcAb),在ELISA检测PAIgG影响因素的预试方面以及对一批正常人(N=34)和不同类型的血小板减少症患者(N=65)进行了PAIgG测定的比较,结果表明;HRP—McAb和HRP—PcAb在ELISA检测PAIgG法各预试条件方面多数相近似,二种试剂检测值间呈现较好的相关性(Y=0.689,P<0.0005)故在常规ELISA检测PAIgG法中可用HRP—McAb代替HRP—PcAb,以利实验方法的标准化。

THE COMPARISON OF THE MEASUREMENT OF PLATELETASSOCIATED IGG WITH HORSERADISH PEROXIDASE CONJUGATED MONOCLONAL ANTIBODY AND HORSERADISH PEROXIDASE CONJUGATED POLYCONAL ANTIBODY

Using horseradish peroxidase conjugated antihuman IgG monoclonal anti- body(HRP-McAb) and horseradish peroxidase conjugated antihuman IgG(Poly- colonal antibody)(HRP-PcAb), determined platelet associated IgG(PAIgG) and compared the results in the effect on preexperiment with the results of determining PAIgG in some volunteers(N=34) and some various thrombocytopenias(N=65). It has been suggested that HRP-McAb and HRP-PcAb arc simrlar to major preexperimental conditions of determining PAIgG. Both of the reagents showed a better correlation in determining PAIgG (r=0.689. P<0,0005). In order to make the esperiment stardard,. one can use the HRP-McAb in routing ELISA of determining PAIgG to replace HRP-PcAc.