IAA与HRP联合作用对人胰腺癌BXPC-3的细胞毒效应

目的　研究吲哚乙酸(IAA)与辣根过氧化物酶(HRP)在体外共同作用于人胰腺癌BXPC 3 细胞所产生的细胞毒效应。方法　MTT法测定IAA/HRP对BXPC 3细胞增殖的影响;流式细胞仪分析BXPC 3 细胞周期时相变化;胎盘兰(曲利本兰)排斥试验,研究IAA/HRP对BXPC 3 细胞死亡率的影响;原位细胞凋亡检测试剂盒检测IAA/HRP作用后BXPC 3细胞的凋亡。结果　IAA/HRP体外对BXPC 3 细胞有抑制生长的效应,且这种效应有浓度和时间依赖性;BXPC 3细胞在IAA/HRP作用48 h后细胞周期被阻滞在G1期和G2期;IAA/HRP作用72 h后对照组和药物组的凋亡细胞数存在着显著性差异,且凋亡细胞数有浓度依赖性。结论　IAA/HRP对胰腺癌BXPC 3 细胞有细胞毒作用,可体外抑制细胞的增殖,诱导凋亡。

Study of the cytotoxicity of combination of indole-3-acetic(IAA) and horseradish peroxidase(HRP) on BXPC-3 cells in pancreatic cancer

Objective To study the cytotoxicity of combination of indole-3-acetic(IAA) and horseradish peroxidase(HRP) on BXPC-3 cells in pancreatic cancer. Methods Cell proliferation was measured by MTT assay; cell cycle was analyzed by flow cytometry; trypan blue repulsion was used to assay the mortality of BXPC-3 cells treated by IAA/HRP; in Situ Cell Apoptosis Detection Kit was used to detect the apoptosis of BXPC-3 cells. Results IAA/HRP showed significant growth-inhibitive effect in a dose and time-dependant pattern in vitro; the flow cytometry revealed G1 phase and G2 phase arrest in the cell cycle 48 hours after the treatment with IAA/HRP; there were significant differences between the mortality of the control group and that of treatment group at 72 hours; apoptosis cells showed significant difference between the control group and IAA/HRP group. Conclusion The combination of IAA and HRP has cytotoxicity on BXPC-3 cells, and inhibits cell proliferation and induces cell apoptosis in vitro.