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ABC-ELISA法实验过程中影响因素的研究
引用本文:张中健,方亮,谢天益. ABC-ELISA法实验过程中影响因素的研究[J]. 西安交通大学学报(医学版), 1987, 0(4)
作者姓名:张中健  方亮  谢天益
作者单位:西安医科大学病毒学研究室,西安医科大学病毒学研究室,西安医科大学病毒学研究室
摘    要:
本文介绍了用ABC-ELISA法检测HBsAg,较系统地研究了ABC-ELISA法实验过程中的主要影响因素。结果发现:ABC-ELISA法实验过程中的非特异性显色不仅来自抗生物素的非特异性吸附,而且还来自生物素化抗体的非特异性吸附。本文提出了消除ABC-ELISA法实验过程中非特异性显色的切实可行的方法。


INVESTIGATION OF FACTORS AFFECTING THE PERFORMANCE OF ABC-ELISA
Zhang Zhong jian,. INVESTIGATION OF FACTORS AFFECTING THE PERFORMANCE OF ABC-ELISA[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1987, 0(4)
Authors:Zhang Zhong jian  
Affiliation:Research Laboratory of Virology
Abstract:
This paper deals with the examination of HBsAg with ABC-ELISA, The major factors affecting the performance of ABCELISA were studied more systematically. We found that non-specific stain in the performance of ABC-ELISA was caused not only by non-specific adhesion of avidin but also by non-specific adhesion of biotinylated antibody, A feasible method to reduce non-specific stain in the performance of ABC-ELISA was suggested in this paper.
Keywords:biotin  avidin  ABC-ELISA  HBsAg  factors affecting the performance of ABC-ELISA
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