| 预缺血对培养乳鼠心肌细胞缺血再灌注损伤的延迟保护作用 |
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| 引用本文： | 赵健,黄庆恒,范朝晖,郑建杰.预缺血对培养乳鼠心肌细胞缺血再灌注损伤的延迟保护作用[J].西安交通大学学报(医学版),1999(1). |
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| 作者姓名： | 赵健 黄庆恒 范朝晖 郑建杰 |
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| 作者单位： | 第一临床医学院心胸外科!西安７１００６１ |
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| 摘 要： | 观察了预缺血对培养乳鼠心肌细胞缺血再灌注损伤的影响。结果发现，经预缺血诱导的，Ｃ肌细胞超氧化物歧化酶（ＳＯＤ）活性升高，与缺血再灌注组（Ｉ－Ｒ）比较，经典预适应组（ＣＰＣ）和延迟预适应组（ＬＰＣ）乳酸脱氢酶（ＬＤＨ）释放减少，心肌细胞存活率升高，脂质过氧化减轻，膜流动性增加。提示：培养乳鼠心肌细胞存在延迟预适应，其机制与预缺血提高心肌细胞抗氧化酶的活性、增加缺血再灌注时自由基的清除有关。
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| 关 键 词： | 细胞培养 预适应 缺血再灌注 |
THE PROTECTIVE EFFECTS OF ISCHEMIC PRECONDITIONING AGAINST ISCHEMIA REPERFUSION INJURY IN CULTURED MYOCYTES OF INFANT RAT |
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| Zhao Jian, Huang Qingheng, Fan Zhaohui et al.THE PROTECTIVE EFFECTS OF ISCHEMIC PRECONDITIONING AGAINST ISCHEMIA REPERFUSION INJURY IN CULTURED MYOCYTES OF INFANT RAT[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1999(1). |
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| Authors: | Zhao Jian Huang Qingheng Fan Zhaohui |
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| Abstract: | We observed the protective effects ofischemic preconditioning against ischemia reperfusion injury in cultured myocytes of infant rat. Theresults indicated that superoxide dismutase (SOD)activity increased significantly in transient group.Compared with ischemia reperfusion group (I-Rgroup), LDH release decreased and myocytes servival ratio increased significantly in classic preconditioning group (CPC group) and late preconditioninggroup (LPC group). At the same time, lipid peroxide decreased and plasma membrane fluidity increased significantly. It suggests: ischemic preconditioning has protective effects gaginst ischemiareperfusion in cultured myocytes of infant rail Onemajor mechanism may be that ischemic preconditioning increases myocyte antioxidase activity and improves the possibility of eliminating the free radicalin ischemia reper fusion. |
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| Keywords: | cultured myocyte ischemic preconditioningl ischemia reper fusion |
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