| 体外诱导软骨细胞形成的实验方法研究 |
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| 引用本文： | 史增敦,张美丽,莫东旭.体外诱导软骨细胞形成的实验方法研究[J].西安交通大学学报(医学版),1990(3). |
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| 作者姓名： | 史增敦 张美丽 莫东旭 |
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| 作者单位： | 西安医科大学地方性骨病研究所
(史增敦，张美丽)，西安医科大学地方性骨病研究所(莫东旭) |
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| 摘 要： | 本文用19日～21日胎龄的SD种胎鼠的后肢肌肉与同种大鼠骨基质明胶一起做体外诱导培养,培非液为含3 700 mg/L NaHCO_3的DMEM。培养10日时,8个培养物中有7个出现诱导性软骨细胞。培养15、20、25日时,30个培养物(每个时间点10个培养物)中都出现了诱导性软骨细胞。结果表明,本实验所采用的组织培养方法和含3 700 mg/L NaHCO_3的DMEM培养液完全适用于体外诱导软骨细胞形成。
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| 关 键 词： | 骨基质明胶 胎鼠肌肉 组织培养 软骨细胞 |
STUDY ON THE EXPERIMENTAL CONDITIONS OF BMG-INDUCED CHONDROCYTE FORMATION IN VITRO |
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| Shi Zengdun,et al.STUDY ON THE EXPERIMENTAL CONDITIONS OF BMG-INDUCED CHONDROCYTE FORMATION IN VITRO[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1990(3). |
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| Authors: | Shi Zengdun |
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| Institution: | Institute of endemic bone diseases |
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| Abstract: | Thigh skeletal muscle of 19d～21d(near-term) fetal rat cultured together with bone matrix gelatin (BMG) in vitro. The medium was DMEM containing 3700 mg/L NaCHO_3. After 1O d in culture, there were BMG-induced chondrocytes in 7 out of 8 explants. After 15、20 and 25 d in culture. BMG-induced chondrocytes were observed in all of the 30 expiants(10 explants in each point of time-period). The result shows that the experimental conditions and culture medium used in this paper were suitable for the system of BMG-induced choudrocyte formation in vitro. |
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| Keywords: | BMG fetal rat musele tissue culture chondrocyte |
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