硒对胎鼠肝细胞核酸及蛋白质合成的影响

本文报道了微量元素硒对培养纯种Wistar大白鼠胎肝细胞核酸及蛋白质合成的影响。观察了肝细胞培养基中不同硒水平对~3H-TdR(~3H-脱氧胸苷)、~(14)C-UR(~(14)C-尿苷)参入率的影响,同时还测定了培养基中白蛋白及甲胎蛋白(AFP)的含量。结果显示:细胞培养基中硒(Se)浓度在0.4～0.6μg/ml范围内使~3H-TdR、~(14)C-UR的参入率、白蛋白及甲胎蛋白含量均明显高于不补硒组。说明硒在这一浓度范围内能促进肝细胞DNA、RNA更新率及蛋白质合成。

THE EFFECT OF SELENIUM ON THE NUCLEIC ACID AND PROTEIN SYNTHESIS OF THE CULTURING FETAL RAT LIVER CELLS

In order to determine the effect of trace elementselenium on dcoxyribonucleic acid (DNA), ribonucleicacid (RNA) and protein synthesis in culturing fetal rathepatocytes, the incroporation of ~3H-deoxythymidine(~3H-TdR) into DNA, ~(14)C-uridine (~(14)C--UR) intoRNA by fetal rat hepatocytes cultured inselenium--supplemented culture medium were investigated In addition the amounts of albumin and fetalprotein released from the same hepatocytes into the cul-ture medium were also determined. It was shown thatthe incorporation rate of ~3H--TdR into DNA and~(14)C--UR into RNA by fetal hepatocytes cultured in themedium supplemented with selenium as selenite at arang of Q 4 to Q 6 μg per ml levels were significantlyhigher than those cultured in medium withoutselenium--supplementation, concomitantly the amountsof albumin and--fetal protein released into the sameselenium--supplemented culture medium were alsogreater signicantly than those did in the mediumwithout selenium--supplementaion (as control). Theresults suggest that selenium supplementation in culturemedium at the levels of Q 4 to Q 6 μg selenium per mlcan promote the synthesis of DNA, RNA and proteinby fetal rat hepatocytes