The cloning and expression of renalase and the preparation of its monoclonal antibody |
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| Authors: | Feng Wang Nian-song Wang Tao Xing Yang Cao Hai-yan Xiang |
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| Institution: | (1) Department of Nephrology, the 6th People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, China;(2) the Australian School of Advanced Medicine, Macquarie University, Sydney, NSW, Australia;(3) School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, China |
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| Abstract: | To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified
from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones,
the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare
the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which
can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum
renalase.
Foundation item: the Scientific Research Project of Shanghai Municipal Health Bureau (2008Y034), and the Natural Scientific Research Project
of Shanghai (05ZR14086) |
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| Keywords: | renalase expression monoclonal antibody |
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