| 5-Aza-CdR对人食管癌细胞株生物学行为及TFPI-2 mRNA表达的影响 |
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| 引用本文： | 杜雅冰,邵应举,樊青霞,孙桢,王琳,赵培荣.5-Aza-CdR对人食管癌细胞株生物学行为及TFPI-2 mRNA表达的影响[J].西安交通大学学报(医学版),2011,32(3):295-298. |
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| 作者姓名： | 杜雅冰 邵应举 樊青霞 孙桢 王琳 赵培荣 |
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| 作者单位： | 郑州大学第一附属医院肿瘤内科,河南郑州,450002 |
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| 摘 要： | 目的探讨5-氮杂-2-脱氧胞苷(5-Aza-2-deoxycytidine,5-Aza-CdR)干预对食管鳞癌细胞株Eca9706生长增殖及其组织因子途径抑制物2(tissue factor pathway inhibitor-2,TFPI-2)基因表达的影响。方法选取食管鳞癌细胞株Eca9706,并用不同浓度的5-Aza-CdR处理该细胞株。MTT法检测干预前后细胞增长率的变化,FCM法检测干预前后细胞凋亡率的变化,RT-PCR技术检测干预前后Eca9706细胞TFPI-2基因mRNA的表达,免疫组化检测干预前后Eca9706细胞TFPI-2蛋白的表达。结果食管鳞癌细胞株Eca9706存在TFPI-2基因超甲基化状态,经5-Aza-CdR处理后,超甲基化状态解除,细胞增殖受到抑制,细胞凋亡率明显提高(P<0.05);细胞中TFPI-2蛋白表达明显增强,同时mRNA的表达水平也较处理前明显提高(P<0.05)。结论食管癌细胞系TFPI-2基因超甲基化可抑制其mR-NA表达,当其超甲基化解除后,细胞增殖受到抑制,同时细胞凋亡率相应提高。
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| 关 键 词： | 食管癌 组织因子途径抑制物-2 5-氮杂-2-脱氧胞苷 甲基化 免疫组化 逆转录聚合酶链反应 凋亡 |
Effects of 5-Aza-2-deoxycytidine on biological behavior of esophageal carcinoma cell line and TFPI-2 mRNA expression |
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| DU Ya-bing,SHAO Ying-ju,FAN Qing-xia,SUN Zhen,WANG Lin,ZHAO Pei-rong.Effects of 5-Aza-2-deoxycytidine on biological behavior of esophageal carcinoma cell line and TFPI-2 mRNA expression[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(3):295-298. |
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| Authors: | DU Ya-bing SHAO Ying-ju FAN Qing-xia SUN Zhen WANG Lin ZHAO Pei-rong |
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| Institution: | DU Ya-bing,SHAO Ying-ju,FAN Qing-xia,SUN Zhen,WANG Lin,ZHAO Pei-rong (Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | Objective To explore the effects of 5-Aza-2-deoxycytidine(5-Aza-CdR) intervention on the growth and proliferation of Eca9706 cell line and protein expression of tissue factor pathway inhibitor-2(TFPI-2).Methods Eca9706 cell line was treated by 5-azaCdR of different concentrations;MTT,flow cytometry,immunohistochemistry and RT-PCR were used to determine the cell growth,apoptosis,and the expression of TFPI-2 gene and its protein.Results Eca9706 cell line had hypermethylation of TFPI-2.After demethylation by 5... |
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| Keywords: | esophageal carcinoma tissue factor pathway inhibitor-2 5-Aza-CdR methylation immunohistochemistry RT-PCR apoptosis |
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