| 大鼠急性脊髓损伤中caspase-1的表达及意义 |
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| 引用本文： | 杜恒,戴星,马巍,刘淼.大鼠急性脊髓损伤中caspase-1的表达及意义[J].西安交通大学学报(医学版),2011,32(1):62-64. |
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| 作者姓名： | 杜恒 戴星 马巍 刘淼 |
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| 作者单位： | 西安交通大学医学院第一附属医院骨科,陕西西安,710061 |
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| 摘 要： | 目的研究半胱氨酸天冬氨酸特异性蛋白酶-1(cysteinyl aspartate specific protease-1,caspase-1)在大鼠急性脊髓损伤后的表达及意义。方法取健康成熟SD大鼠36只,随机分为对照组和损伤组,各18只,每组再分8 h、3 d7、d共3个时间点,每个时间点6只。对照组仅做单纯T8、T9椎板切除术,损伤组则按Nystrom法建立大鼠急性脊髓损伤动物模型。HE染色观察脊髓组织病理学变化,免疫组化测定各时间点caspase-1的表达变化。结果对照组大鼠脊髓组织中少量细胞caspase-1表达阳性。脊髓损伤后8 h时,损伤组caspase-1表达高于对照组,3 d时表达最高,7 d时略有降低,但仍高于对照组。脊髓损伤组各时间点caspase-1表达阳性细胞数较对照组明显升高(P<0.01)。结论大鼠脊髓损伤后caspase-1的表达迅速增强,可能是导致脊髓继发性损伤的因素之一。
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| 关 键 词： | 脊髓损伤 凋亡 半胱氨酸天冬氨酸特异性蛋白酶-1 |
The expression of caspase-1 after acute spinal cord injury in rats |
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| DU Heng,DAI Xing,MA Wei,LIU Miao.The expression of caspase-1 after acute spinal cord injury in rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(1):62-64. |
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| Authors: | DU Heng DAI Xing MA Wei LIU Miao |
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| Institution: | DU Heng,DAI Xing,MA Wei,LIU Miao(Department of Orthopedic Surgery,the First Affiliated Hospital,Medical School ofXi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective To investigate the expression of cysteinyl aspartate specific protease-1(caspase-1) after acute spinal cord injury in rats and its significance.Methods Totally 36 healthy adult SD rats were randomly divided into the injury group and the control group.Each group was further divided into 3 time points: 8h,3d and 7d,6 rats were sacrificed at each time point.The control group only underwent T8 and T9 laminectomy without injury.In the injury group the model of acute spinal cord injury was induced with ... |
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| Keywords: | spinal cord injury apoptosis caspase-1 |
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