| ABC—ELISA法检测自身免疫性甲状腺病患者TRAb的研究 |
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| 引用本文： | 何岚,高慧.ABC—ELISA法检测自身免疫性甲状腺病患者TRAb的研究[J].西安交通大学学报(医学版),1992(1). |
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| 作者姓名： | 何岚 高慧 |
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| 作者单位： | 西安医科大学第一附属医院内科学教研室，西安医科大学第一附属医院内科学教研室 西安 710061 研究生，西安 710061 |
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| 摘 要： | 本文叙述了在自身免疫性甲状腺病TRAb检测中引入一种新方法-ABC-ELISA法的研究过程。并将该法与常规ELISA法的检测灵敏度作了比较,用FRTL-5细胞测定TSAb的方法鉴定了该法的可靠法。未治甲亢用ABC—ELISA法测TRAb的阳性率91%,用常规ELISA法测TRAb阳性率70%,用FRTL-5细胞检测了未治甲亢中26例的TSAb所得结果与ABC法相同。据此,作者认为ABC—ELISA是一种敏感性高、重复性好、简便、适用于临床的方法。
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| 关 键 词： | 亲和素-生物素辣根过氧化物酶复合物 促甲状腺激素受体抗体 鼠甲状腺细胞株 格雷夫斯病 甲状腺刺激抗体 甲状腺刺激阻断抗体 |
STUDY OF SERUM TRAb IN AUTOIMMUNE THYROID DISEASE WITH ABC- ELISA |
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| He Lan,Gao Hui.STUDY OF SERUM TRAb IN AUTOIMMUNE THYROID DISEASE WITH ABC- ELISA[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1992(1). |
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| Authors: | He Lan Gao Hui |
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| Abstract: | In this paper the investigation of using a new method-ABC-ELISA in assay of Autoimmune Thyroid Disease are presented. The sensitivity of ABC-ELISA is compared with that of standard ELISA; Its reliability is proven by the methods of detecting TSAb with FRTL-5. TRAb is detected by ABC-ELISA in 91% of untreated Graves'. TRAb is detected by Standard ELISA in 70% of untreated Graves'. The results of ABC-ELISA in 26 untreated Graves' are equal to that of the method of detecting TSAb with FRTL-5. Therefore, we consider that ABC-ELISA is a sensitive, reproducible, convenient method applicable to clinical practice. |
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| Keywords: | Avidin-Biotin-Peroxidase Comples(ABC) TSH recepor Antibody(TRAb) Fischor rat thyroid line-5(FRTL-5) Graves' Disease(GD) Thyroid Stimulating Antibody(TSAb) Thyroid Stimulating Blocking Antibody(TSBAb) |
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