猪骨胶原蛋白降压成分的提取与生物活性的研究

目的　从猪骨胶原蛋白中提取小肽做为血管紧张素转换酶抑制剂。方法　猪骨胶原蛋白经胰蛋白酶水解 ,阳离子交换树脂层析 ,Sephadex G2 5凝胶过滤 ,反相高效液相层析 (HPLC) ,最终得到一种 9肽 ,其氨基酸组成 :Ile,Ser,His,Gly ,Ala,Pro ,Tyr,Leu ,Asp。结果　以马尿酰 甘氨酰 甘氨酸 (HGG)为底物 ,对从猪肺中提取的血管紧张素转换酶 (ACE)有明显的抑制作用 ,I50为 2 .6× 1 0 - 2 mmol/L。将含此肽 0 .0 1 %的混合物做静脉注射试验 ,对肾性高血压大鼠和自发性高血压大白鼠均有明显降压作用 ;口服给药 1 5d ,对自发性高血压有一定的降压作用。对离体豚鼠回肠平滑肌有收缩作用。结论　胶原蛋白酶解物 9肽的抗高血压效果 ,可能是通过对血管紧张素转换酶的抑制作用。

Purification and biological activity of antihypertensive pepti de from collagen

Objective To purify the inhibitor of Angiotensin-converting enzyme in hog bone collagen. Methods The trypsin hydrolysate of hog bone collagen was processed through a cation-exchange column chromatograph,gel filtration sephadex G-25, and successive several reverse phase high performance liquid chromatography(HPLC). The final peptide results was composed of Ile,His,Ser,Gly,Ala,Pro,Tyr,Leu and Asp.Results I 50 value of the nonapeptide for angiolensin-converting enzyme from hog lung was calculated to be approximately 2.6×10 -2 mmol·L -1 with Hip-Gly-Gly as substrate.Intravenous administration of the peptides mixture contained 0.01% the nonapeptide showed that it had antihypertensive effect on Spontaneously and renal hypertensive rats. After oral administration of the mixture for 15 days, the blood pressure of the spontaneous hypertensive rats decreased.The nonapeptide could contract the isolated ileum smooth muscle of guinea pig.Conclusion The antihypertensive effect on renal hypertensive is probably because of the inhibition of ACE.