| 用碱性裂解法提取人血凝块中基因组DNA |
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| 引用本文： | 王小卫,张克洲,熊咏民.用碱性裂解法提取人血凝块中基因组DNA[J].西安交通大学学报(医学版),2007,28(5):592-594. |
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| 作者姓名： | 王小卫 张克洲 熊咏民 |
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| 作者单位： | 1. 西安交通大学教育部环境与疾病相关基因重点实验室,环境与地方病研究室,陕西西安,710061
2. 西安阎良630风湿病医院,陕西西安,710089 |
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| 基金项目： | 国家自然科学基金;陕西省科技攻关项目 |
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| 摘 要： | 目的建立一种新的从人血凝块中提取基因组DNA的方法。方法血凝块于室温自然解冻,匀浆,碱消化,酚氯仿抽提,电泳检测。结果用碱性裂解法从人血凝块中成功提取到基因组DNA,提取的DNA质量浓度平均为0.46 g/L,且吸光度比值A260/A280>1.8,选取人Gpx-1基因作PCR获得满意的目的片段。结论用碱性裂解法提取的基因组DNA的总量较高,提取效率高,PCR扩增效果好,可很好的应用于血凝块基因组DNA的提取。
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| 关 键 词： | 血凝块 DNA提取 碱性裂解法 |
| 文章编号： | 1671-8259（2007）05-0592-03 |
| 修稿时间： | 2007-03-09 |
An alkaline lysis method for extracting genomic DNA from human blood clot |
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| Wang Xiaowei,Zhang Kezhou,Xiong Yongmin.An alkaline lysis method for extracting genomic DNA from human blood clot[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2007,28(5):592-594. |
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| Authors: | Wang Xiaowei Zhang Kezhou Xiong Yongmin |
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| Abstract: | Objective To explore a new method to isolate genomic DNA from human blood clot.Methods Blood clot was thawed naturally in room temperature and homogenized,then digested with alkaline and extracted by phenol and chloroform,electrophoresis detection.Results The genomic DNA was successfully extracted by alkaline lysis method.The average quantity of the extracted DNA was 0.46g/L,while A260/A280>1.8.PCR was performed using the human Gpx-1 gene,and we obtained the needed target gene fragments.Conclusion The alkaline lysis extraction method is reliable for obtaining high quantities of DNA from blood clot suited for PCR amplification. |
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| Keywords: | blood clot DNA extraction alkaline lysis method |
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