CB和MMP-9在上皮性卵巢肿瘤中的表达及相关性研究

目的检测组织蛋白酶B(cathepsin B,CB)与基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在人类上皮性卵巢肿瘤中的表达,探讨两者与卵巢癌细胞的分化增殖、侵袭转移的关系。方法采用链霉素亲和生物素-过氧化物酶法(SP)进行免疫组织化学染色,用CB抗体、MMP-9抗体标记上皮性卵巢肿瘤组织标本,综合染色强度和阳性细胞占总体细胞数的百分比进行染色判定,结合有关病理特征进行分析。结果经统计学分析,CB、MMP-9在上皮性卵巢癌中的表达与年龄、病理类型无相关性(P>0.05),而与卵巢癌的临床分期、有无转移及腹水呈正相关(P<0.05);CB与卵巢癌的组织学分级呈正相关(P<0.05);上皮性卵巢癌中CB和MMP-9表达呈正相关(r=0.652,P<0.05)。结论CB、MMP-9与卵巢癌的恶性生物学行为密切相关,两者在其侵袭转移过程中发挥了重要的协同作用。

Study of expression and correlation of cathepsin B and matrix metallo- proteinase -9 in epithelial ovarian tumor

Objective To study the expression of CB and MMP-9 in ovarian tumor with the histo-differentiation and invasion and metastasis.Methods The expressions of CB and MMP-9 were detected by S-P immunohistochemistry method in histological section of ovarian tumor,and the relationships between the results and the clinical pathological parameters of the patients were assessed.Results The expression of CB and MMP-9 had marked correlation(P<0.05) with clinical stage,metastasis and ascites of the ovarian cancer.Moreover,the expression of CB had marked correlation(P<0.05) with pathologic grade.The positive correlation was observed between the expression of CB and MMP-9(r=0.652,P<0.05).Conclusion The expression of CB and MMP-9 in ovarian cancer cells is closely related to the tumor malignancy biological behaviour of ovarian cancer.They play an important synergistic action in the invasion and metastasis of ovarian cancer.