犬急性肝衰模型的建立

目的　建立犬急性肝衰模型 ,以进一步探讨应用体外异种全肝灌注治疗急性肝衰的方法与效果。方法　选 7只犬予以戊巴比妥钠 2 5mg·kg-1腹腔注射麻醉 ,分别行一期门腔端侧分流 ,肝周韧带断离 ,膈动脉缝扎。肝动脉血流完全阻断 2h ,制备致死性肝衰模型。术中定时采血测ALT、AST、LDH、PT、FIB、ammonia。取犬肝组织行光镜、电镜观察。结果　 1只犬因麻醉过量致死 ,其余 6只均手术成功。所有犬均在全肝血流完全阻断后 (3～7.5h)死亡 ,平均生存时间 (5 .7± 1.2 )h。随全肝血流阻断时间延长 ,动物血ALT、AST、ammonia、LDH进行性升高 ,PT时间延长 ,PIB减少 ,血压进行性下降。结论　一期门腔分流、全肝血流完全阻断 2h制备犬急性肝衰模型方法简便、可靠。

Construction of canine acute liver failure model

Objective To study the therapeutic effect of extracorporeal liver perfusion on the treatment of acute liver failure by constructing canine acute failure model. Methods Seven Mongrol dogs were selected, and anesthesia was induced by injection of barbital sodium (25mg·kg -1). Hepatic failure was induced by an end-to-side portacaval shunt, and then we excised all hepatic connecting ligaments except the hepatic arteries. The common hepatic and gastroduodenal arteries were occluded for 2 hours. The blood levels of ammonia, ALT, AST, LDH, BUN, TBIL, PT and FIB were monitored. Results One dog died of anesthesia, while the other 6 hepatic failure models were successfully induced. After the occlusion of liver blood flow, all dogs died within 3～7.5 hours. The average survival time was 5.7±1.2 hours. Blood levels of ALT, AST, LDH, and ammonia increased significantly. Conclusion Portacaval shunt combined with hepatic artery occlusion for 2 hours can be an effective way to induce canine acute hepatic failure.