CD44V6,Bcl-2在宫颈癌中的表达及临床意义

目的　探讨黏附分子CD44V6和原癌基因Bcl 2在宫颈癌中的表达及临床意义。方法　应用免疫组化S P法检测 153例宫颈癌、正常宫颈上皮组织和宫颈不典型增生 (CIN)中CD44V6和Bcl 2的表达 ,并分析其与临床病理间关系。结果　①CD44V6表达在宫颈癌中最高 ,其次为CIN ,在正常宫颈组织最低 ,差异有显著性 (P <0 .0 5) ;随临床分期越晚 ,组织学分级越高 (CD44V6)表达率越高 ,并与组织学分级显著相关 (P <0 .0 5) ;淋巴结转移组 (CD44V6)表达率明显高于无淋巴结转移组 (P <0 .0 5) ;②Bcl 2表达在CIN中最高 ,其次宫颈癌 ,在正常宫颈组织不表达 ,差异有显著性 (P <0 .0 5) ;Bcl 2表达与临床分期、组织学分级呈负相关 ,且差异均有显著性 (P <0 .0 5) ;淋巴结转移组Bcl 2表达率低于无淋巴结转移组但差异无显著性 (P >0 .0 5)。③CD44V6和Bcl 2在宫颈癌中表达不相关。结论　CD44V6、Bcl 2表达与宫颈癌的发生 ,发展及转移密切相关 ,可作为预测宫颈癌预后的辅助指标

The expression and significant of CD44V6 and Bcl-2 in cervical carcinoma

Objective To investigate the expression of CD44V6 and Bcl-2 in cervical carcinoma and clinical significant in the progress of th is type of cancer. Methods A total of 153 cases of cervical carcinoma, normal cervical epithelium and cervical dysplasia were investigated for the expression of CD44V6 and Bcl-2 with immunohistochemistry SP method. Results ① The positive rate of CD44V6 was 69.44% in cervical carcinoma, 45.45% in cervical dysplasia and 8.33% in normal cervix epithelium ( P < 0.05 ). The expression was positively correlated with clinical stages( P > 0.05 )and pathological grades( P < 0.05 ). CD44V6 staining was rema rkably higher in the group with positive lymph nodes than in the group without ( P < 0.05 ). ② The positive rate of Bcl-2 was 49.07% in cervical carci noma and 60.61% in cervical dysplasia and there was no expression in the normal cervix epithelium ( P < 0.05 ). Bcl-2 staining in cervical cancer with path ological grades and clinical stages was found to be decreased, and the expressio n in grades and stages showed remarkably significant differences ( P < 0.05 ). Bcl-2 staining was lower in the group with positive lymph nodes than in the group without. ③ There was no relationship between expression of CD44V6 and Bcl -2 ( P > 0.05 ).Conclusion The overexpression of CD44V6 and Bcl-2 may be l inked to the pathogenesis, development and metastasis of cervical carcinoma. Det ermination of CD44V6 and Bcl-2 may be useful for properly estimating the progno sis of cervical carcinoma.