| 丙型肝炎病毒核心蛋白基因序列变异分析 |
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| 引用本文： | 郭晏海,赵君庸,苏成芝,阎小君,肖乐义.丙型肝炎病毒核心蛋白基因序列变异分析[J].西安交通大学学报(医学版),1999(1). |
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| 作者姓名： | 郭晏海 赵君庸 苏成芝 阎小君 肖乐义 |
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| 作者单位： | 生物化学教研室!西安７１００６１(郭晏海，赵君庸)，第四军医大学基因诊断技术应用研究所!西安(苏成芝，阎小君，肖乐义) |
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| 摘 要： | 为了研究西安地区丙型肝炎病毒（ＨＣＶ）基因组中Ｃ区基因的变异性，采用单链构象多态性（Ｓｉｎｇｌｅ－ＳｔｒａｎｄＣｏｎｆｏｒｍａｔｉｏｎＰｏｌｒｍｏｒｐｈｉｓｍ，ＳＳＣＰ）分析万法，对３６份ＨＣＶＲＮＡ阳性血清的ＰＣＲ产物进行分析，结果有３１份样品的ＳＳＣＰ图谱相同，对其中二份样品进行测序，证明序列是相同的；另外５价样品ＳＳＣＰ图谱不相同，其序列与前者也有差异。说明本地区ＨＣＶ基因组中Ｃ区基因存在一个主要的基因类型。该实验为本地区ＨＣＶ诊断试剂和疫苗的研究确定了研究材料。
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| 关 键 词： | HCV 核蛋白基因 序列变异分析 SSCP |
SEQUENCE VARIABILITY ANALYSIS OF THE HCV CORE PROTEIN GENE |
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| Guo Yanhai, Zhao Junyong, Su Chengzhi et al.SEQUENCE VARIABILITY ANALYSIS OF THE HCV CORE PROTEIN GENE[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1999(1). |
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| Authors: | Guo Yanhai Zhao Junyong Su Chengzhi |
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| Institution: | Department of Biochemistry |
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| Abstract: | in order to research the variability ofHCV core protein gene (HCV C gene) in Xi'an, weanalyzed 36 pieces of serum's PCR products of HCVgene by SSCP. The results showed that 31 sampleshad the same SSCP graph. We sequenced 2 PCRproducts of the 31 and found out that their se-quences were the same. The other 5 samples' SSCPgraphs were different from the 31 samples. We sequenced one PCR product of the 5 and the resultwas different from the 2 samples' we had sequenced. It proves that there is a main type of HCVC gene in Xi'an. The experiment points out a way toresearch the diagnosis kit and vaccine of HCV inXi'an. |
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| Keywords: | HCV core protein gene sequence analysis SSCP |
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