吗啡中毒大鼠死后体内吗啡再分布的影响因素

目的　确定大鼠生前摄入吗啡量、死亡时间及各组织pH值在死后吗啡再分布中的作用。方法　建立急、慢性吗啡中毒大鼠模型 ,测定死后 0～ 96h心血、心肌、肝、肾、脑组织吗啡含量 ,用pH计测定各检材的 pH值 ,并与相应检材的吗啡含量变化进行相关分析。结果　①吗啡急性中毒 (一次肌肉注射 10mg·kg- 1 )组和慢性中毒 (累计 7d肌肉注射吗啡总量 6 4 5mg·kg- 1 )组大鼠死后同一组织吗啡含量变化差异显著 ,如脑、肝、肾组织 ;②死后 0～ 96h吗啡中毒大鼠不同时间段 ,同一组织中的吗啡含量发生显著改变 ,如心血、心肌、肝组织、肾组织 ;③心血、肝、肾组织的pH值与该检材中的吗啡含量变化呈负相关。结论　吗啡中毒大鼠死后体内吗啡再分布受大鼠生前摄入吗啡量、死亡时间及组织pH值的影响

Factors affecting the postmortem redistribution of morphine in poisoned rats

Objective To determine how the internal amount of morphine before death, time of death and pH of the tissues are responsible for postmortem redistribution of morphine in poisoned rats. Methods The model of acute and chronic morphine poisoning in rats was established. Samples were examined for free morphine concentration at 0-96?h after death. Samples were examined for pH at various internals, and regression analysis of the amount of morphine in the tissues at corresponding time was made. Results ① The changes of the same tissues between the two groups were compared at the same time. The difference was significant in the brain, the liver and the kidneys; ② The amount of morphine significantly changed in the cardiac blood and various tissues (apart from brain tissue in the acute poisoning group) at 0-96h after death; ③ A significant negative correlation was found between increase in tissue or blood morphine concentration and decreased pH. Conclusion The internal amount of morphine before death, time of death and pH of tissue significantly affect the postmortem redistribution of morphine in rats.