| 高效液相色谱法测定胆汁中结合胆汁酸 |
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| 引用本文： | 王亚文,石景森.高效液相色谱法测定胆汁中结合胆汁酸[J].西安交通大学学报(医学版),1990(2). |
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| 作者姓名： | 王亚文 石景森 |
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| 作者单位： | 西安医科大学测试中心
(王亚文)，西安医科大学第一附属医院肝胆外科研究室(石景森) |
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| 摘 要： | 报道反相高效液相色谱法测定人胆汁中结合胆汁酸,样品处理手续简便,因为色谱系统使用了保护柱。回收率范围是87.01%～110.75%,检出限为0.09～0.21μg,用本方法分析胆石症患者及对照组胆汁结合胆汁酸。
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| 关 键 词： | 胆汁 结合胆汁酸 胆石 色谱 |
DETERMINATION OF CONJUGATED BILE ACIDS IN BILE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| Wang Yawen.DETERMINATION OF CONJUGATED BILE ACIDS IN BILE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1990(2). |
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| Authors: | Wang Yawen |
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| Abstract: | A reversed-phase high performance liquid chromatographic method for the determination of conjugated bile acids in human bile had been reported. The procedure of sample preparation is simple since a guard column is used in this system. Recovery of the added bile salts was in the range of 87.01～110.75%. The limit of detection ranged from 0.09 μg to 0.21 μg. The method is used for the analysis of conjugated bile acids of bile from the patients with gallstone and control groups. |
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| Keywords: | chromatography bile conjugated bile acids gallstone |
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