细胞凋亡与桥本氏甲状腺炎发病相关研究

目的　探讨细胞凋亡及其相关抗原Fas、FasL和Bcl 2与桥本氏甲状腺炎发病的关系。方法　采用免疫组化法和原位末端标记技术对 8例桥本氏甲状腺炎 (HT)和 8例正常对照甲状腺组织中Bcl 2、Fas、FasL和细胞凋亡情况 ,进行了检测和光密度测定分析。结果　①HT组中滤泡细胞强烈表达Fas和FasL ,并且细胞凋亡率高 ,与之相反 ,正常对照组轻度表达Fas ,不表达FasL ,同时有少量细胞凋亡 (P <0 .0 0 1)。②Bcl 2在HT组中弱表达。正常对照组中Bcl 2强表达 (P <0 .0 5 )。结论　桥本氏甲状腺炎中甲状腺细胞通过上调Fas、FasL及下调Bcl 2的表达而促进细胞凋亡 ,故细胞凋亡在桥本氏甲状腺炎的发病机制中起一定作用

Apoptosis and the pathogenesis of Hashimoto's thyrioditis

Objective To clarify the relationship between apoptosis and the pathogenesis of Hashimoto's thyroiditis (HT). Methods The expressions of Bcl-2, Fas, FasL and the apoptosis in 8 cases of HT and 8 cases of normal control were investigated by using immunohistochemical and TdT-mediated dUTP nick end labeling techniques. Results ①Thyroid follicles in HT samples were strongly stained by Fas and FasL, with the high percentage of apoptosis (P<0.001). But only moderate expression of Fas was found in normal controls, with very few apoptosis (P<0.05). ②Immunostaining for Bcl-2 was weak in HT, while high in normal (P<0.001). Conclusion ①Apoptosis may play a role in the pathogenesis of HT. The apoptosis was prompted by up-regulating the expression of Fas, FasL and down-regulating of Bcl-2 in HT.