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| 散癖平胃方对人胃癌细胞SGC-7901侵袭转移的影响 | |
| 引用本文： | 党晓燕,董蕾,史海涛,秦勇.散癖平胃方对人胃癌细胞SGC-7901侵袭转移的影响[J].西安交通大学学报(医学版),2011,32(6):778-782. |
| 作者姓名： | 党晓燕 董蕾 史海涛 秦勇 |
| 作者单位： | 1. 西安交通大学医学院第二附属医院消化内科,陕西西安710004;西安交通大学医学院第二附属医院急诊科,陕西西安710004 2. 西安交通大学医学院第二附属医院消化内科,陕西西安,710004 3. 西安市中心医院普二科,陕西西安,710004 |
| 基金项目： | 陕西省13115重大科技专项课题(No.2007ZDKG-73)~~ |
| 摘 要： | 目的观察散癖平胃(SPPW)方对人胃癌细胞SGC-7901侵袭转移能力的影响。方法 SPPW方含药血清干预人胃癌细胞SGC-7901 48h后,应用黏附实验、侵袭实验及迁移实验,检测SPPW方对人胃癌细胞SGC-7901侵袭转移能力的影响。应用实时定量聚合酶链反应(real-time PCR)检测SPPW对与侵袭转移有关的MMP-2和MMP-9mRNA表达的影响。结果 SPPW方各组含药血清干预人胃癌细胞48h后,SGC-7901细胞黏附抑制率、侵袭抑制率及迁移抑制率均增加且呈剂量依赖关系,与阴性对照组比较有统计学差异(P<0.01或P<0.05)。SPPW方三个剂量组对MMP-2mRNA的表达无统计学差异,对MMP-9mRNA的表达具有统计学差异(P<0.01)。结论 SPPW方可抑制人胃癌细胞SGC-7901黏附、侵袭和迁移能力,其抑制有明显的剂量依赖性,其抑制的机制可能与下调MMP-9的表达有关。 |
| 关 键 词： | 散癖平胃方 胃癌细胞SGC-7901 黏附 侵袭 转移 MMP-2 MMP-9 |
Effects of Sanpipingwei formula on invasion and metastasis of gastric cancer SGC-7901cells in vitro |
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| DANG Xiao-yan,DONG Lei,SHI Hai-tao,QIN Yong.Effects of Sanpipingwei formula on invasion and metastasis of gastric cancer SGC-7901cells in vitro[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(6):778-782. | |
| Authors: | DANG Xiao-yan DONG Lei SHI Hai-tao QIN Yong |
| Institution: | DANG Xiao-yan1,2,DONG Lei1,SHI Hai-tao1,QIN Yong3(1.Department of Gastroenterology,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710004,2.Department of Emergency,3.Department of General Surgery,Xi'an Central Hospital,China) |
| Abstract: | Objective To investigate the effects of Sanpipingwei Formula(SPPW) on invasion and metastasis of gastric cancer SGC-7901 cells in vitro.Methods After 48-hour treatment with serum containing traditional Chinese medicine SPPW,the effect on adhesion of SGC-7901 cells was tested by cell matrigel adhesion assay.Transwell chamber assay was performed to determine the effect on invasion and migratory capacity of the cells.The level of MMP-2 and MMP-9 mRNA in the cells was measured with real-time PCR.Results After 4... |
| Keywords: | Sanpipingwei Formula SGC-7901 cell adhesion invasion metastasis MMP-2 MMP-9 |
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