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| 三硝基苯磺酸/乙醇灌肠液诱导大鼠溃疡性结肠炎TLR4/NF-κB和PI3K/AKT/NF-κB信号通路的表达及电针的干预作用 | |
| 引用本文： | 张超贤,郭李柯,郭晓凤.三硝基苯磺酸/乙醇灌肠液诱导大鼠溃疡性结肠炎TLR4/NF-κB和PI3K/AKT/NF-κB信号通路的表达及电针的干预作用[J].西安交通大学学报(医学版),2015(2):263-270. |
| 作者姓名： | 张超贤 郭李柯 郭晓凤 |
| 作者单位： | 1. 新乡医学院第一附属医院消化内科,河南卫辉,453100 2. 新乡医学院第一附属医院口腔内科,河南卫辉,453100 3. 杭州市第六人民医院肝病科,浙江杭州,310014 |
| 摘 要： | 目的探讨三硝基苯磺酸(TNBS)/乙醇灌肠液诱导大鼠溃疡性结肠炎TLR4/NF-κB和PI3K/AKT/NF-κB信号通路的表达及电针的干预作用。方法雄性Wistar大鼠240只,随机分为正常对照组、模型组、电针组、TLR4单克隆抗体(TLR4mAb)组、LY294002组和TLR4mAb-LY294002联合治疗(T&L)组。以TNBS/乙醇溶液灌肠诱导大鼠溃疡性结肠炎模型,电针组造模同时给予电针足三里穴治疗,TLR4mAb组给予TLR4mAb腹腔注射,LY294002组则予LY294002注射液腹腔注射,T&L组则同时给与上述TLR4mAb和LY294002腹腔注射,共4周,每日行疾病活动指数(DAI)评分。造模4周后处死大鼠取结肠组织标本,评价结肠黏膜损伤指数(CMDI)和组织学损伤指数(TDI);免疫印迹法测定结肠黏膜磷酸化AKT(P-AKT)和活化NF-κB含量;RT-PCR法检测结肠组织中TLR4mRNA、PI3K mRNA、AKT mRNA、NF-κB mRNA、TNF-αmRNA及IL-1βmRNA表达。结果与正常对照组比较,模型组结肠组织TLR4mRNA、PI3K mRNA、P-AKT、活化NF-κB、TNF-αmRNA、IL-1βmRNA表达和DAI、CMDI及TDI均显著升高(P<0.01);与模型组比较,TLR4mAb组TLR4mRNA表达显著下降(P<0.01),LY294002组PI3K mRNA、P-AKT表达显著下降(P<0.01),而在电针组和T&L组TLR4mRNA、PI3K mRNA及P-AKT表达均明显降低(P<0.01);与上述变化相对应,与模型组相比,各治疗组活化NF-κB、TNF-αmRNA、IL-1βmRNA表达和DAI、CMDI及TDI均明显降低(P<0.05或P<0.01),电针组和T&L组上述6指标优于TLR4mAb组和LY294002组,活化NF-κB与TNF-αmRNA及IL-1βmRNA表达呈正相关(r1=0.579,P<0.05;r2=0.561,P<0.05)。结论电针足三里穴能显著降低实验性溃疡性结肠炎大鼠的NF-κB活性及TNF-αmRNA、IL-1βmRNA表达,从而减轻结肠组织损害程度,这与其阻断TLR4/NF-κB和PI3K/AKT/NF-κB信号通路有密切关系。 |
| 关 键 词： | 电针 溃疡性结肠炎 toll样受体4(TLR4)NF-κB信号通路 PI3K/AKT/NF-κB信号通路 活化核转录因子-κB 肿瘤坏死因子-αmRNA IL-1βmRNA |
The expressions of Toll-like receptor4/NF-κB and PI3K/AKT/NF-κB signaling pathways in ratulcerative colitis induced by the combined enema of trinitrobenzene sulphonic acid and ethano and the interventional effect of electroacupuncture on them |
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| ZHANG Chao-xian,GUO Li-Ke,GUO Xiao-feng.The expressions of Toll-like receptor4/NF-κB and PI3K/AKT/NF-κB signaling pathways in ratulcerative colitis induced by the combined enema of trinitrobenzene sulphonic acid and ethano and the interventional effect of electroacupuncture on them[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2015(2):263-270. | |
| Authors: | ZHANG Chao-xian GUO Li-Ke GUO Xiao-feng |
| Institution: | ZHANG Chao-xian;GUO Li-Ke;GUO Xiao-feng;Department of Gastroenterology,the First Affiliated Hospital of Xinxiang Medical University;Department of Stomatology,the First Affiliated Hospital of Xinxiang Medical University;Department of Hepatology,the Sixth People’s Hospital of Hangzhou; |
| Abstract: | |
| Keywords: | electroacupuncture ulcerative colitis toll-like receptor 4 (TLR4 )/NF-ΚB signaling pathway PI3K/AKT/NF-κB signaling pathway active NF-κB TNF-αmRNA IL-1βmRNA |
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