| RLC－310人体肾透明细胞癌细胞株的生物学和形态学特点 |
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| 引用本文： | 陈葳,李旭,杨少毅,杨玉琮,程晓丽,阎春芳.RLC－310人体肾透明细胞癌细胞株的生物学和形态学特点[J].西安交通大学学报(医学版),1996(4). |
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| 作者姓名： | 陈葳 李旭 杨少毅 杨玉琮 程晓丽 阎春芳 |
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| 作者单位： | Molecular Biology Center，First Affiliated Hospital |
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| 摘 要： | 对新建的克隆性人肾透明细胞癌细胞株ＲＬＣ－３１０进行了生物学鉴定和形态学观察。发现细胞可重叠生长，群体倍增时间３２ｈ，集落形成率４４％，具有超二倍体核型，染色体众数６４。细胞没有支原体污染，有特异性的ＬＤＨ同功酶谱，细胞接种Ｂａｌｂ／ｃ裸鼠可形成移植瘤。细胞大小均一，核大，细胞表面有丰富的微绒毛，未见到细胞间的桥粒结构。胞质内有变形的粗面内质网、线粒体和较多糖原颗粒，张力原纤维可见。细胞核异形性明显，核仁数目增多，有边集。表明这株细胞保持了肾透明细胞癌的基本特点。
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| 关 键 词： | 肾细胞癌，细胞株，生物学，形态学 |
BIOLOGICAL AND MORPHOLOGICAL CHARACTERISTIOS OF A HUMAN RENAL CARCINOMA CELL LINE(RLC-310) |
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| Chen Wei, Li Xu, Yang Shaoyi, et al.BIOLOGICAL AND MORPHOLOGICAL CHARACTERISTIOS OF A HUMAN RENAL CARCINOMA CELL LINE(RLC-310)[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1996(4). |
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| Authors: | Chen Wei Li Xu Yang Shaoyi |
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| Abstract: | A newly established renal carcinoma cell line derived from a human renal clear cell carcinoma of kidney, designated as RLC-310, was observed biologically and morpholgically. The cells had a doubling time of 32-hour and a 44% colony formation rate in soft agar,The karyotype showed a hyperdiploid feature with a modal number of 64.The cells remained free of mycoplasma and had a distionct lactic acid dehydrogenase isozyme pattern.The cells could produce tumors in athymic nude mice. Numerous microvilli were present in the cells and desmosomes were not observed. Rough endoplasmic reticulum, mitochondria and glycogen aggregates were seen.A pleiomorphic nucleus with marginated nucleoli was seen in all cells. |
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| Keywords: | renal carcinoma cell line biology morphology |
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