改进并应用双电极埋植技术记录大鼠胃肠肌电活动

目的　改进并应用双电极埋植技术记录大鼠的胃肠肌电活动。方法　选用外径仅 0 .5mm的聚四氟乙烯薄膜包绕的 7股银丝绝缘导线制作记录电极 ,采用套针埋植电极。结果　共埋植双电极 160对 (每只大鼠 1～ 5对 ) ,约 1周时 ,15 4对 ( 96.3 % )电极可持续有效地记录胃肠肌电活动 ,5周后仍有 12 3对电极 ( 76.9% )可正常工作。结论　改进的肌电记录法具有以下优点 :①方法简便可行 ,成功率高 ;②对研究对象损伤轻微 ,在小动物及人体上均适用 ;③可同时埋植多对电极 ,记录多处肌电活动 ;④电极工作稳定持久 ,可行慢性试验 ,动态观察接近生理状态下的胃肠肌电活动

Improvement of the technique of bipolar electrode implantation for gastrointestinal myoelectrical recordings in rats

Objective To improve the technique of bipolar electrode implantation for myoeletrical recordings in rats. Methods We chosed teflon-coated seven-strand silver thread wire with outer diameter of only 0.5?mm to make recording electrode, and used injection needles as a trocar to implant the electrode in the bowel wall. Results We implanted a total of 160 pairs of bipolar electrodes into the bowels of rats. About a week later, 154 pairs of electrodes (96.3%) continuously functioned allowing repeated gastrointestinal myoeletrical recordings. 123 electrodes(76.9%)worked well over 5 weeks. Conclusion Our technique was a great improvement over the old one and its advantages include: ①There is little technical difficulty and high success rate in implantation; ②There is little damage on the subjects and can be used in many small animals and humans; ③Many pairs of electrodes implantation and many places myoelectrical recordings can be made at the same time; ④It has long term durability, observation of gastrointestinal myoeletrical activity near physiological state continuously.