Fas和Bcl-2蛋白在人脑星形细胞瘤中的表达及意义

目的　研究Fas及Bcl 2蛋白在人脑星形细胞瘤中的表达特点和相互关系 ,为预后评估及治疗提供理论依据。方法　收集人脑星形细胞瘤标本 5 1例 ,非肿瘤患者的正常脑组织 10例。标本均行HE染色与SP免疫组织化学染色。分析Fas及Bcl 2蛋白表达程度与星形细胞瘤恶性分级的关系。结果　正常脑组织未见Fas蛋白表达 ,只有 1例表达Bcl 2蛋白 ,且表达程度非常弱。星形细胞瘤均有Fas及Bcl 2蛋白的表达 ,并且表达率及表达强度随着星形细胞瘤级别的增高而增强。Fas蛋白表达阳性的肿瘤中Bcl 2蛋白表达率 (6 8.6 % )显著高于Fas蛋白表达阴性的肿瘤 (43.8% )。结论　Fas和Bcl 2蛋白可能与星形细胞瘤的发生和发展有较密切的关系 ,且它们在星形细胞瘤的发生中可能存在相互作用的关系。Fas及Bcl 2蛋白在星形细胞瘤中的表达 ,对疾病预后评估及针对性免疫治疗具有一定的临床价值。

Expression and significance of Fas and Bcl-2 proteins in human astrocytoma

Objective To study the characteristics of Fas and Bcl-2 protein expression in human astrocytoma and their relations so as to provide a theoretical basis for prognostic evaluation and clinical therapy. Methods The expression of Fas and Bcl-2 proteins was investigated by SP immunohistochemical technique in 51 cases of astrocytoma specimens and 10 cases of normal brain tissues. All of the specimens were stained by HE, and the relationship between expression intensity of Fas/Bcl-2 proteins and malignancy grade (WHO gradeⅠ,Ⅱ,Ⅲ, and Ⅳ) of astrocytomas was analyzed. Results The normal brain tissues did not show Fas protein immunostaining, and showed very weak Bcl-2 protein immunostaining in one case. By contrast, the astrocytomas showed Fas and Bcl-2 proteins immunostaining, and their positive rates and the intensity of expression were higher in high-grade astrocytomas than those in low-grade ones. There was also obvious difference between normal brain tissues and astrocytoma groups. In the Fas positive cases, Bcl-2 had a higher positive rate (68.6%) compared with that of Fas negative cases (43.8%). Conclusion Fas and Bcl-2 protein may be involved in tumoriogenesis and development of astrocytomas, and they might have a mutual effect on each other. Our results suggest that the expression of Fas and Bcl-2 in astrocytoma have some clinical values in prognostic evaluation and immunotherapy.