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| 原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系 | |
| 引用本文： | 宋玉华,宋三泰,江泽飞,钱露,陈立勇,郭宁.原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系[J].西安交通大学学报(医学版),2008,29(2):171-175. |
| 作者姓名： | 宋玉华 宋三泰 江泽飞 钱露 陈立勇 郭宁 |
| 作者单位： | 1. 青岛大学医学院附属医院肿瘤科,山东青岛,266031;军事医学科学院基础医学研究所,北京,100850 2. 军事医学科学院附属医院,北京,100071 3. 军事医学科学院基础医学研究所,北京,100850 |
| 摘 要： | 目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA,通过RT-PCR扩增Fra-1全长基因,将其克隆入pcDNA3.1(-)myc-his表达载体;应用脂质体法转染MCF-7细胞,观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移,在肿瘤细胞的侵袭和转移过程中发挥作用。 |
| 关 键 词： | 乳腺癌 Fra-1 AP-1 肿瘤转移 重组质粒 转染 |
| 文章编号： | 1671-8259(2008)02-0171-05 |
| 修稿时间： | 2007年6月19日 |
Association of overexpression of proto-oneogene Fra-1 with the metastatic phenotype of breast cancer cells |
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| Song Yuhua,Song Santai,Jiang Zefei,Qian Lu,Chen Liyong,Guo Ning.Association of overexpression of proto-oneogene Fra-1 with the metastatic phenotype of breast cancer cells[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2008,29(2):171-175. | |
| Authors: | Song Yuhua Song Santai Jiang Zefei Qian Lu Chen Liyong Guo Ning |
| Abstract: | Objective To illustrate the association of Fra-1 overexpression with motility and invasiveness of breast cancer cells.Methods Fra-1 gene was obtained by RT-PCR.PCR products were cloned into the plasmid pcDNA3.1(-) myc-his to create the recombinant plasmid pcDNA3.1(-) myc-his/Fra-1.The plasmid was then transfected into human breast cancer cell line MCF-7.The proliferation,adhesion and migration of MCF-7 cells stably overexpressing Fra-1 were investigated.Results The enhanced growth,adhesion and migration of MCF-7 cells overexpressing Fra-1 were observed.Conclusion Invasiveness and metastasis of tumor cells could be promoted by Fra-1 mediated enhancement of adhesion and migration of tumor cells. |
| Keywords: | breast cancer Fra-1 AP-1 metastasis recombinant plasmid transfection |
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