| 人卵巢癌多药耐药细胞系COC_1/DDP的耐药机制探讨 |
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| 引用本文： | 任娟,张明慧,陈葳,李旭.人卵巢癌多药耐药细胞系COC_1/DDP的耐药机制探讨[J].西安交通大学学报(医学版),1998(3). |
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| 作者姓名： | 任娟 张明慧 陈葳 李旭 |
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| 作者单位： | 西安第一临床医学院肿瘤医院放射治疗科!西安(任娟)，710061(张明慧)，西安第一临床医学院临床分子生物学中心(陈葳，李旭) |
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| 摘 要： | 用体外药物连续选择法建立了一株人卵巢癌多药耐药细胞系。发现耐药细胞内谷胱甘肽含量、谷胱甘肽过氧化物酶活性、谷胱甘肽转硫酶活性均增加，从而使细胞内药物失活增加；细胞内药物作用靶减少，拓扑异构酶活性降低；细胞内药物积聚量减少，但未见P-糖蛋白过度表达。提示卵巢癌耐药性的产生与药物失活增加、药物作用靶减少及细胞内药物积聚量减少有关。
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| 关 键 词： | 人卵巢癌细胞系 多药耐药性 药物失活 DNA拓扑异构酶 P-糖蛋白 |
THE CHARACTERIZATION IN MULTIDRUG RESISTANT MECHANISMS OF A HUMAN OVARIAN CARCINOMA DRUG RESISTANT CELL LINE COC_1/DDP |
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| Ren Juan, Zhang Minghui, Chen Wei et al.THE CHARACTERIZATION IN MULTIDRUG RESISTANT MECHANISMS OF A HUMAN OVARIAN CARCINOMA DRUG RESISTANT CELL LINE COC_1/DDP[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1998(3). |
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| Authors: | Ren Juan Zhang Minghui Chen Wei |
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| Institution: | Radiotherapy Department of Tumor Hospital |
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| Abstract: | in order to study the multidrug resistant phenomena, we established a human ovariancarcinoma MDR cell line COC1/DDP in vitro by progressive drug selection. We analyzed the drug resistant mechanisms and detected that the amount ofglutathione, the activity of glutathione peroxidaseand glutathione -S-transferase in COC1 /DDP cellswere all obviously higher than those of the parentalcell COC1, which resulted in more drug inactivation. The activity of DNA topoisomerase was reduced. The cellular drug accumulation was reducedand there was no P-glycoprotein expressed in COC1/DDP cells. These results indicate that MDR phenomena are related to the addition of drug inactivation, the reduction of drug,s target and cellular drugaccumulation. |
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| Keywords: | human ovarian carcinoma cell line multidrug resistance drug inactivation DNA topoisomerase P-glycoprotein |
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