脑梗塞患者血浆同型半胱氨酸水平与叶酸、B_(12)的关系

目的　研究同型半胱氨酸 (HCY)与脑梗塞的关系及叶酸、维生素B1 2 (B1 2 )对HCY水平的影响。方法　采用高效液相色谱荧光检测法测定 40例脑梗塞患者和 2 0例同龄健康对照组血浆HCY浓度 ,用SimulTRAC SNB放射法测定两组血清叶酸、B1 2 的含量。结果　①脑梗塞患者血浆HCY浓度 (2 4 0 2 μmol/L± 1 1 2 4 μmol/L)高于对照组 (1 4 0 μmol/L± 4 48μmol/L) (P <0 0 1 ) ;②脑梗塞患者叶酸水平 (4 82 μg/L± 1 97μg/L)显著低于对照组 (6 88μg/L± 2 85μg/L) (P <0 0 0 1 ) ;脑梗塞患者B1 2 含量 (2 4 7 56ng/L± 1 58 72ng/L)略低于对照组 (2 70 31ng/L±1 98 39ng/L) ,但无显著差异 (P >0 0 5) ,Pearson相关分析显示两组HCY浓度与叶酸水平均呈负相关性 ,与B1 2 水平无显著相关。结论　脑梗塞患者血浆HCY浓度高于同龄健康人 ;HCY浓度与叶酸水平呈负相关性 ,与B1 2 水平相关性不显著

Plasma homocysteine to folate and vitamin B_(12) in cerebral infarction

To investigate the plasma homocysteine level and cerebral infarction and the association between plasma homocysteine level and levels of serum folate and vitamin B12.Methods　Levels of plasma homocysteine of 40 cerebral infarction patients and 20 healthy subjects were determined using high-performance liquid chromatography with fluorescence detection. Serum folate and vitamin B12concentrations were measured by SimulTRAC-SNB radioassay.Results　①The mean plasma level of homocystine was higher in patients than that in control［(24.02±11.24)*!μmol/L versus(14.10±4.48)*!μmol/L，P＜0.01］;②The level of serum folate was significantly lower in patients than that in control［(4.82±1.97)*!μg/L versus(6.88±2.85)*!μg/L,P＜0.001］.The level of serum B12was slightly lower in patients［(247.56±158.72)*!ng/L］ than that in control［(270.31±198.39)*!ng/L］,but the difference was not statistically significant (P＞0.05).Person correlation anslysis revealed that plasma homocysteine level showed a strong inverse association with folate level in patients(r=-0.39，P=0.01)and in controls(r=-0.46，P=0.03).Vitamin B12 did not show correlation to plasma homocysteine in cases or controls(P＞0.05).Conclusion　①Plasma homocysteine level in cerebral infarction patients is higher than that in the same age health.②There is a strong inverse association between plasma homocysteine and folate.The association between plasma homocysteine and vitamin B12 is not statistically significant.