| GROWTH DIFFERENTIATION FACTOR-5 STIMULATES THE GROWTH AND ANABOLIC METABOLISM OF ARTICULAR CHONDROCYTES |
| |
| 作者姓名： | 许鹏 郭雄 张银刚 Jung Park Klaus von der Mark |
| |
| 作者单位： | Institute of Endemic Diseases，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Institute of Endemic Diseases，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Institute of Endemic Diseases，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Institute of Endemic Diseases，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Institute of Endemic Diseases，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China |
| |
| 基金项目： | ThisworkwassupportedbyNationalNaturalScienceFoundationofChina(No.30400163) |
| |
| 摘 要： | Growthdifferentiationfactor5(GDF5),also knownascartilage derivedmorphogeneticprotein1(CDMP1),isamemberofthebonemorphogenet icprotein(BMP)familywhichbelongstothe transforminggrowthfactorβ(TGFβ)superfami ly1].GDF5isarelativelynewmemberofthe BMPfamily.LikeotherBMPs,implantationofre combinantGDF5caninduceectopiccartilagefor mationinmusculartissues.Thephysiologicalrole ofGDF5hasbeenreportedincludingregulationof myogenesis,regulationofchondrogenesis,bone morphogenesis,andneurondifferen…
|
| 关 键 词： | 细胞 生长生理 老鼠 关节 GDF |
| 文章编号： | 1671-8267（2005）02-0188-06 |
GROWTH DIFFERENTIATION FACTOR-5 STIMULATES THE GROWTH AND ANABOLIC METABOLISM OF ARTICULAR CHONDROCYTES |
| |
| Xu Peng;Guo Xiong;Zhang YinGang;Jung;Park;Klaus;von;der;Mark.GROWTH DIFFERENTIATION FACTOR-5 STIMULATES THE GROWTH AND ANABOLIC METABOLISM OF ARTICULAR CHONDROCYTES[J].Academic Journal of Xi’an Jiaotong University,2005,17(2):188-192. |
| |
| Authors: | Xu Peng;Guo Xiong;Zhang YinGang;Jung;Park;Klaus;von;der;Mark |
| |
| Institution: | Xi'an Jiaotong University |
| |
| Abstract: | Objective To observe the effect of growth differentiation factor-5 (GDF-5) on the growth and anabolic metabolism of articular chondrocytes. Methods The articular chondrocytes isolated from rats were treated with various concentrations of rmGDF-5, and the growth of chondrocytes measured by MTT assay, the cellular cartilage matrices formation detected sulfated glycosaminoglycan by Alcian blue staining and type Ⅱcollagen by RT-PCR. Results After 7 days culture, MTT assay showed that GDF-5 enhanced the growth of chondrocytes in a dose-dependent manner, RT-PCR showed that GDF-5 clearly induced the synthesis of type Ⅱ collagen because of the col2a1 mRNA band more and more strong in a dose-dependent. Chondrocytes were cultured with GDF-5 for 14 days, the intensity of Alcian blue staining was greatly enhanced, especially, at a high concentration of blue-stainable material in a concentration-dependent manner and in a does-dependent manner. Conclusion GDF-5 enhanced the growth of mature articular chondrocytes, and stimulated the cellular cartilage matrices formation in mono-layer culture. |
| |
| Keywords: | growth differentiation Factor-5 articular chondrocytes cell growth matrix formation rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
