| STUDY ON DIFFERENTIATION OF RATS EMBRYONIC STEM CELLS CULTURED IN BRL-CM INTO NEURAL PRECURSOR CELLS |
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| 作者姓名： | 张晓智 李旭 徐海伟 陈葳 |
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| 作者单位： | Central Laboratory，Central Laboratory，Department of Physiology，Central Laboratory First Hospital of Xi'an Jiaotong University，Xi'an 710061，China，First Hospital of Xi'an Jiaotong University，Xi'an 710061，China，Third Military Medical University，First Hospital of Xi'an Jiaotong University，Xi'an 710061，China |
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| 基金项目： | ThisworkwassupportedbytheFoundationofScience&TechniqueInvestigationProjectofShaanxiProvince(No .2 0 0 2K10 G4) |
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| 摘 要： | Embryonicstem (ES)cellsrefertothosesepa ratedfromtheinteriorcellmassthathasnotyetim bedded .Thesetotipotentialcellsareinhighundif ferentiatedstate ,canbecultivatedandproliferateinvitro1] .Underinductioninvitro ,EScellscanbedifferentiatedintocellsthatbelongtothreeembry oniclayers2 ] ,suchasmyocardiumcells ,bloodcellsandneuralcells .GeneticoperationscanbedonewithoutlosingfeaturesoftheEScells.Thereforeithasbecomethemaintoolintheup to datedcelltherapy .Becausetheyarelikelytoformteratomawhentr…
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STUDY ON DIFFERENTIATION OF RATS EMBRYONIC STEM CELLS CULTURED IN BRL-CM INTO NEURAL PRECURSOR CELLS |
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| Zhang Xiaozhi,Li Xu,Xu Haiwei ,Chen Wei Central Laboratory,First Hospital of Xi'an Jiaotong University,Xi'an ,China..STUDY ON DIFFERENTIATION OF RATS EMBRYONIC STEM CELLS CULTURED IN BRL-CM INTO NEURAL PRECURSOR CELLS[J].Academic Journal of Xi’an Jiaotong University,2003,15(1). |
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| Authors: | Zhang Xiaozhi Li Xu Xu Haiwei Chen Wei Central Laboratory First Hospital of Xi'an Jiaotong University Xi'an China |
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| Institution: | 1. Central Laboratory, First Hospital of Xi'an Jiaotong University, Xi'an 710061,China
2. Department of Physiology, Third Military Medical University |
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| Abstract: | Objective To investigate whether buffalo rat liver cell conditioned medium (BRL CM) can be used as the culture medium of embryonic stem (ES) cells, and to get relatively pure neural precursor cells (NPCs) for treatment aim. Methods Mouse ES cells were cultured in BRL CM and medium contain leukemia inhibitory factor (LIF), respectively. NPCs were selectively cultured in serum free medium. Alkaline phosphatase activity was visualized with NBT/BCIP and nestin antigen was detected with immunocytochemical methods. Results BRL CM could be used as an efficiency culture condition instead of LIF in ES cells culture. About 86% of cells derived from ES cells in the serum free culture were NPCs. Conclusion BRL CM can replace LIF to use in ES cell culture. High purity of NPC can be induced from ES cells with serum free culture method. |
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| Keywords: | embryonic stem cell neural precursor cell cell culture |
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