STUDY OF ECK GENE EXON-3 FROM HUMAN NORMAL TISSUE AND BREAST CANCER CELL LINE |
| |
作者姓名: | 李瑶琛 孔令洪 王一理 司履生 |
| |
作者单位: | Institute For Cancer Research,Institute For Cancer Research,Institute For Cancer Research,Institute For Cancer Research School of Life Science & Technology,Xi'an Jiaotong University,Xi'an 710061,China,School of Life Science & Technology,Xi'an Jiaotong University,Xi'an 710061,China,School of Life Science & Technology,Xi'an Jiaotong University,Xi'an 710061,China,School of Life Science & Technology,Xi'an Jiaotong University,Xi'an 710061,China |
| |
摘 要: | ECK (epithelialcellkinase) ,alsonamedasEphA 2 ,isoneofthemembersofEphAsubfamilyinEphfamily .ECK protein ,atransmembranety rosinekinase1] ,canbestructurallydividedintothreedomainsaccordingtotheirpositionsincells.ThreedomainsareexternaldomainwithanN ter minalsignalpeptide ,atransmembranedomainandacytoplasmicdomainwhichincludesacanonicalpro tein tyrosinekinasecatalyticdomain .ECKiswide lyexpressedinavarietyofhumantissues ,abun dantly presentinlung ,skin ,smallintestineandovary .ECKisalsoe…
|
STUDY OF ECK GENE EXON-3 FROM HUMAN NORMAL TISSUE AND BREAST CANCER CELL LINE |
| |
Abstract: | Objective To establish a method cloning the exon 3 of eck gene from normal tissue and ZR-75-1 cell line (a human breast cancer cell line)and study whether these genes exist mutant. Methods Designed a pair of specific primers and amplified the exon 3 of eck gene fragment from the extracted genomic DNA derived from normal epithelial cells from skin tissue and ZR-75-1 cell line respectively by PCR technique. Transformed the E.coil. JM109 with recombinant plamids constructed by inserting the amplified fragments into medium vector pUCm-T and sequenced these amplified fragments after primary screening of endonuclease restriction digestion and PCR amplification. Results ① Obtained the genomic DNA of human normal epithelial cells and ZR-75-1 cell line respectively. ② Obtained the amplified fragments of human exon 3 of eck gene through PCR technique. ③ Obtained the cloning vectors of exon 3 of eck gene of human normal epithelial cells and ZR-75-1 cell line respectively. ④ ZR-75-1 cell line exists mutation of nucleotides. Conclusion Successfully established the method of cloning the human exon 3 of eck gene and found some mutations in the detected samples. This study lays a foundation for further studying the function of eck gene in tumorgenesis. |
| |
Keywords: | eck(ephA2) gene gene cloning polymerase chain reaction p53 protein ZR 75 1 cell line |
本文献已被 CNKI 万方数据 等数据库收录! |