| EFFECT OF ANGIOTENSIN Ⅱ RECEPTOR SUBTYPE ⅠON THE FIRING RATE IN CATECHOLAMINERGIC TUMOR CELLS |
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| 引用本文： | 杜剑青,孙成文,唐敬师,Colin Sumners,Mohan K Raizada.EFFECT OF ANGIOTENSIN Ⅱ RECEPTOR SUBTYPE ⅠON THE FIRING RATE IN CATECHOLAMINERGIC TUMOR CELLS[J].西安交通大学学报(英文版),2003,15(2). |
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| 作者姓名： | 杜剑青 孙成文 唐敬师 Colin Sumners Mohan K Raizada |
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| 作者单位： | Department of Physiology，Medical School of Xi’an Jiaotong University，Department of Physiology，College of Medicine，University of Florida，Department of Physiology，Medical School of Xi’an Jiaotong University，Department of Physiology，College of Medicine，University of Florida，Department of Physiology，College of Medicine，University of Florida Xi’an 710061，China，U.S.A.，Xi’an 710061，China，U.S.A.，U.S.A. |
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| 摘 要： | AngiotensinⅡ (AngⅡ ) playsanimportantroleintheregulationofbloodpressure1 ] .Theac tionofAngⅡismediatedmainlybystimulationoftheangiotensintype 1(AT1 )receptorandinvolvesintheactivityofcatecholaminergicsystem .Im munostainingstudieshaveindicatedthattheAT1 re ceptorexistsonthecatecholaminergicneurons2 ] .AdministrationofAngⅡintorat’sbrainincreasedtheconcentrationsofdopamine ,epinephrine ,andnorepinephrineinthebrain3 ] .Althoughanatomicalandfunctionalevidencessupporttheinteractionbe twe…
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EFFECT OF ANGIOTENSIN Ⅱ RECEPTOR SUBTYPE Ⅰ ON THE FIRING RATE IN CATECHOLAMINERGIC TUMOR CELLS |
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| Colin Sumners,Mohan K Raizada.EFFECT OF ANGIOTENSIN Ⅱ RECEPTOR SUBTYPE Ⅰ ON THE FIRING RATE IN CATECHOLAMINERGIC TUMOR CELLS[J].Academic Journal of Xi’an Jiaotong University,2003,15(2). |
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| Authors: | Colin Sumners Mohan K Raizada |
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| Abstract: | Objective To study the action of brain angiotensin Ⅱ(Ang Ⅱ) receptors and underlying intracellular mechanism in the catecholaminergic system(CATH) Methods Action potentials (APs) of the primary co-cultured catecholaminergic tumor (CATH.a) cells were recorded with the whole-cell patch clamp configuration in current clamp mode. Expression of Ang Ⅱ receptors subtypes (AT1 and AT2) was detected by RT-PCR technique. Results The differentiated CATH.a cells represented a neuron-like characterization. All CATH.a cells expressed mRNA encoding both Ang Ⅱ AT1 and AT2 receptor subtypes. Ang Ⅱ increased the firing rate in the CATH.a cells, which was inhibited completely by addition administration of the AT1 but not AT2 receptor antagonist, and partially by using the inhibitors of signal molecules, U73122 (10 μmol*L-1), or KN-93 (10 μmol*L-1), or calphostin C (10 μmol*L-1). Conclusion Ang Ⅱ increases firing rate in CATH.a cells via AT1 receptor. The CATH.a cells expressing functional AT1 and AT2 receptor subtypes may be of general utility for the study of the Ang Ⅱ receptor-induced modulation of brain catecholaminergic system. |
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| Keywords: | angiotensin II CATH a cell AT 1 receptor cell firing spark |
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