| CHONDROCYTE APOPTOSIS IN ARTICULAR CARTILAGE WITH KASHIN-BECK DISEASE |
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| 作者姓名： | 王世捷 郭雄 左弘 许鹏 平智广 张银刚 张增铁 耿冬 |
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| 作者单位： | Department of Imaging Center，Second Hospital of Xi anJiaotong University，Xi an710004，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China，Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University)，Ministry of Education，Xi'an 710061，China |
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| 基金项目： | ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(No.39970663)andNaturalScienceFounda tionofXi anJiaotongUniversity(No.XJJ 2004133). |
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| 摘 要： | Kashin BeckDisease(KBD)isachronic,en demicosteoarthritisaffectingover0.81millionspa tientsand101millionsofpeopleatriskinChina1]. Thebasicpathologicalfeaturesofthediseaseis chondrocytesdegenerationandnecrosisinthedeep zoneofarticularcartilageandgrowthpl…
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| 关 键 词： | 凋亡 肌醇胺 关节软骨 Kashin-Beck病 KBD |
CHONDROCYTE APOPTOSIS IN ARTICULAR CARTILAGE WITH KASHIN-BECK DISEASE |
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| Wang Shijie,GUO Xiong,Zuo Hong,Xu Peng,Ping Zhiguang,ZHANG Yingang,Zhang Zengtie,Geng Dong.CHONDROCYTE APOPTOSIS IN ARTICULAR CARTILAGE WITH KASHIN-BECK DISEASE[J].Academic Journal of Xi’an Jiaotong University,2005,17(1):80-85. |
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| Authors: | Wang Shijie GUO Xiong Zuo Hong Xu Peng Ping Zhiguang ZHANG Yingang Zhang Zengtie Geng Dong |
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| Institution: | 1. Department of Imaging Center, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China
2. Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an 710061, China |
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| Abstract: | Objective To investigate the apoptosis of articular chondrocyte and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage with Kashin-Beck disease(KBD) in order to understand the pathogenesis of chondronecrosis in KBD. Methods The collected samples of human articular cartilage were divided into two groups: control group (15 samples from 15 cases), KBD group (15 samples from 15 cases). KBD patients were diagnosed by "Pathological Criteria to Diagnose KBD in China". Chondrocyte apoptosis was detected by TUNEL staining, and the Bcl-2, Bax, Fas and iNos positive articular chondrocytes were stained by the B-SA of immunohistochemistry. Articular cartilage was classified three zones and the positive rate were counted by light microscope for cytoplasimic staining by polyclonal antibodies of Bcl-2, Bax, Fas and iNos and apoptotic chondrocytes by TUNEL. Results ① The percentage of positive apoptotic chondrocytes stained by TUNEL in the middle zone of articular cartilage from the KBD-children group(33.60±2.71%) was higher than that of the control (1.33±0.41% t=11.59, g=28, P<0.01). ②The percentage of chondrocytes staining for Bcl-2, Bax, Fas and iNos among the upper and the middle zone in KBD group were significantly higher than that of the control (t=11.75-18.65, g=14, P<0.01); the remarkable difference in the expression of Bcl-2, Bax, Fas and iNos among the upper, the middle and the deep zones was also seen in KBD articular cartilage (F=73.49-114.42, g=42, P<0.01), and staining for Bcl-2, Bax, Fas and iNos in KBD children was prominent in the upper zone(41.93±12.26%, 45.60±15.78%, 53.60±16.49%, 45.47±14.02%) and the middle zone(14.93±3.50%, 13.87±4.32%, 23.27±4.83%, 21.67±6.82%)of articular cartilage, respectively. Conclusion The chondrocyte apoptosis and the present of Bcl-2, Bax, Fas and iNos positive chondrocytes in articular cartilage of children with KBD were significantly higher than that of the control. |
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| Keywords: | Kashin-Beck disease apoptosis bcl-2 bax fas iNos |
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