| Isolation and Characterization of Microorganisms for Degradation of Minimal Oils and Fats at Low Temperatures |
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| 作者姓名： | 陈熹兮 杨虹 李道棠 李堃宝 |
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| 作者单位： | Dept.ofEcologicalandEnvironmentalSciences,CollegeofLifeScienceandTechnology,ShanghaiJiaotongUniv.,Shanghai200240,China |
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| 基金项目： | The Housing Equipment &Environm entFacilities Group，Japan |
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| 摘 要： | The isolation of microorganisms for biodegradation of minimal fats and oils at low temperatures was reported. By using rapeseed oil as a sole carbon source, six strains were isolated from five kinds of oils/fats-contaminated wastewater, soil, and active sludge. Among them, two strains which show the highest oil removal ratios were identified as Pseudomonas pseudoalcaligenes and Pseudornonas mendocina, respectively. The experiments of orthogonal impact conditions show that the optimal oil degradation condition is at pH 8.0, 5 C and 100 mg/L oil. Under this condition, the rapeseed oil degradation ratios of two strains after 24 hours amount to 92. 6% and 92.0% respectively, whereas the removal ratios of lard decrease to 39.5% and 54.3%.
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| 关 键 词： | 菜籽油 猪油 低温 微生物降解 假单胞菌属 假厌氧生物 |
Isolation and Characterization of Microorganisms for Degradation of Minimal Oils and Fats at Low Temperatures |
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| CHEN Xi-xi,YANG Hong,LI Dao-tang,LI Kun-bao.Isolation and Characterization of Microorganisms for Degradation of Minimal Oils and Fats at Low Temperatures[J].Journal of Shanghai Jiaotong university,2003,8(2):123-127. |
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| Authors: | CHEN Xi-xi YANG Hong LI Dao-tang LI Kun-bao |
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| Institution: | Dept. of Ecological and Environmental Sciences, College of Life Science and Technology, Shanghai Jiaotong Univ., Shanghai 200240, China |
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| Abstract: | The isolation of microorganisms for biodegradation of minimal fats and oils at low temperatures was reported. By using rapeseed oil as a sole carbon source, six strains were isolated from five kinds of oils/fats contaminated wastewater, soil, and active sludge. Among them, two strains which show the highest oil removal ratios were identified as Pseudomonas pseudoalcaligenes and Pseudomonas mendocina, respectively. The experiments of orthogonal impact conditions show that the optimal oil degradation condition is at pH 8.0, 5 C and 100 mg/L oil.Under this condition, the rapeseed oil degradation ratios of two strains after 24 hours amount to 92. 6% and 92.0% respectively, whereas the removal ratios of lard decrease to 39.5% and 54.3%. |
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| Keywords: | rapeseed oil lard low temperature bioremediation Pseudomonas pseudoalcaligenes Pseudomonas mendocina |
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