Expression and Application of the Recombinant Non-Structural Proteins of FMDV

Foot-and-mouth disease virus (FMDV) is acausative agent of highly infectious and economi-cally important disease in cloven-hoofed animals.In Asian and South American countries, commer-cial vaccines of FMDV are widely used for control-ling the disease, but animals in North America andEurope are not inoculated with FMD vaccines.Identifying animals that have been infected withFMDV is important because infected cattle andsheep can become carriers of the virus1,2]Althoughonly African buff…

Expression and Application of the Recombinant Non-Structural Proteins of FMDV

FMDV non-structural protein gene(NSPs) 3ABC, 3AB, 2C and 3D were amplified and cloned into expression plasmid pET-32a(+). The recombinant NSPs were produced in E.coli and purified using Ni~(2+) affinity column. Western-blotting indicated that the NSPs were expressed correctly. Using the recombinant NSPs, indirect ELISAs have been set up to distinguish FMDV-infected pigs from vaccinated ones. Experimental results indicate that the immunogenesity of recombinant 3AB protein is strong and can be the ideal antigen for detection; the immunogenecity of 2C is weak and sensitivity of the assay is low; 3D is not an ideal antigen ,for the specificity of assay based on 3D is low.