| 紫贻贝多烯脂肪酸乙酯及牛磺酸的提取与分析 |
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| 引用本文： | 张翼,穆军,吕红丽,顾晓洁,史继诚.紫贻贝多烯脂肪酸乙酯及牛磺酸的提取与分析[J].大连铁道学院学报,2011(2):63-68. |
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| 作者姓名： | 张翼 穆军 吕红丽 顾晓洁 史继诚 |
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| 作者单位： | 大连交通大学环境与化学工程学院; |
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| 基金项目： | 国家自然科学基金资助项目(20902009); 中国科学院实验海洋生物学重点实验室开放基金资助项目(Kf201009); 辽宁省高校优秀人才支持计划资助项目(2009R08); 大连市优秀青年科技人才基金资助项目(2008J23JH041) |
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| 摘 要： | 为综合开发利用低价值贝类中的功能保健品成分,利用索氏/超声提取、尿素包合法提取了紫贻贝中的多烯脂肪酸乙酯,对提油后的残渣利用热水提取、离子交换层析富集的方法提取了其中的牛磺酸,利用高效液相色谱-二极管阵列检测器（HPLC-DAD）分析测定了产品中二十碳五烯酸乙酯（EPA-E）、二十二碳六烯酸乙酯（DHA-E）的含量,利用薄层色谱法、吸光光度法定性定量测定了产品中的牛磺酸,结果显示紫贻贝中含有相当量的多烯脂肪酸乙酯及牛磺酸成分,有望作为高价值功能保健食品的来源.
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| 关 键 词： | 紫贻贝 二十碳五烯酸乙酯 二十二碳六烯酸乙酯 牛磺酸 提取 分析 |
Extraction and Analysis of Polyenoic Fatty Acyl Ethyl Esters and Taurine in Mytilus edulis |
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| ZHANG Yi,MU Jun,Lü Hong-li,GU Xiao-jie,SHI Ji-cheng.Extraction and Analysis of Polyenoic Fatty Acyl Ethyl Esters and Taurine in Mytilus edulis[J].Journal of Dalian Railway Institute,2011(2):63-68. |
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| Authors: | ZHANG Yi MU Jun Lü Hong-li GU Xiao-jie SHI Ji-cheng |
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| Institution: | ZHANG Yi,MU Jun,Lü Hong-li,GU Xiao-jie,SHI Ji-cheng(School of Environmental and Chemical Engineering,Dalian Jiaotong University,Dalian 116028,China) |
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| Abstract: | For comprehensive utilization of the functional health products from the low-value shells,the polyunsaturated fatty acyl ethyl esters in Mytilus edulis were extracted by Soxhlet/supersonic extraction and urea complexation,and taurine in the residue after the fatty extraction was extracted by hot water digestion and ion-exchange chromatography.The contents of eicosapentaenoic acyl ether ester(EPA-E) and docosahexaenoic acyl ethyl esters(DHA-E) in the product were analyzed by HPLC-DAD.By thin layer chromatogr... |
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| Keywords: | Mytilus edulis EPA-E DHA-E taurine extraction analysis |
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