姜黄素对食管癌细胞系放射敏感性的影响及其机理

目的　探讨姜黄素对食管癌细胞系Eca10 9的放射增敏作用及其机制。方法　利用细胞克隆技术进行剂量 -存活曲线分析 ,末端脱氧核苷酰转移酶法检测细胞凋亡 ,并对细胞周期进行分析。结果　姜黄素组D0 值为 91.35cGy ,空白对照和空白药物组D0 值分别为 130 .0 7cGy和 138.37cGy ,(P <0 .0 5 )。流式细胞仪分析空白组和姜黄素组凋亡区阳性细胞分别为 1.0 1%和 87.5 8% (P <0 .0 5 ) ;经姜黄素处理后 ,与对照组相比 ,G0 1 期细胞比例降低 ,G2 + M期细胞比例增高。结论　姜黄素可以明显提高Eca 10 9细胞的放射敏感性 ,机制可能与其引起瘤细胞周期阻滞 ,诱导肿瘤细胞凋亡有关

The influence of curcumin on the radiosensitivity of esophageal carcinoma cell lines and its mechanism

Objective To investigate the influence of curcumin on the radiosensitivity of esophageal carcinoma cell lines (Eca109) and its mechanism. Methods The colony formation ability of Eca109 cells after X-ray irradiation was analyzed by linear-quadratic formulation. The apoptotic percentage was measured by TUNEL and cell cycle was analyzed by FCM. Results The D 0 value of Eca109 cell lines was 97.35?cGy, and that of control group and free-curcumin group was 130.07cGy and 138.37?cGy, respectively (P< 0.05). The apoptotic percentage of Eca109 cell lines exposed to free-medicine and curcumin group was 1.01% and 87.58%, respectively (P< 0.05). In curcumin group the percentage of cells in G 2+M phase increased. Conclusion Curcumin can increase the radiosensitivity of esophageal carcinoma cell lines, which may be associated with blocking cell cycle and inducing apoptosis of Eca109 cells.