| 牛心肌线粒体ADP／ATP载体蛋白的分离及应用 |
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| 引用本文： | 张王刚,林元喜,王一理,王哲训,刘治全.牛心肌线粒体ADP／ATP载体蛋白的分离及应用[J].西安交通大学学报(医学版),1996(2). |
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| 作者姓名： | 张王刚 林元喜 王一理 王哲训 刘治全 |
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| 作者单位： | 西安第二附属医院血液科 |
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| 摘 要： | 将分离的牛心肌线粒体先与其ＡＤＰ／ＡＴＰ载体蛋白抑制剂苍术苷交联，然后用ＴｒｉｔｏｎＸ－１００溶解线粒体膜，经羟基磷灰石离心制得该载体蛋白。用聚丙烯酰胺凝胶电泳及生物学方法鉴定，发现所分离的ＡＤＰ／ＡＴＰ载体蛋白分子量约为３０ＫＤ，可作为抗原用于抗该载体蛋白抗体的研究。
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| 关 键 词： | 牛心肌线粒体，ADP／ATP载体蛋白，分离 |
ISOLATION OF THE ADP/ATP CARRIER FROM BEEF HEART MITOCHONDRIA AND ITS APPLICATION |
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| Zhang Wanggang,Lin Yuanxi,Wang Yili,et aL.ISOLATION OF THE ADP/ATP CARRIER FROM BEEF HEART MITOCHONDRIA AND ITS APPLICATION[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1996(2). |
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| Authors: | Zhang Wanggang Lin Yuanxi Wang Yili et aL |
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| Abstract: | By preluding the beef heart mitochondria with carboxyatractylate (CAT),a stable CAT protein complex could be retained after solubilization with Triton X-100.As purification hydroxyapatite is most effective,the CAT protein complex appears in supernatant after centrifugation, subsequent gel filtration or sepharose results in a 1.5 fold further enrichement of CAT protein.A single band of 30 KD molecular weight is found in sodium dodecyl sulfate polyacrylanide gel electrophoresis.The ADP/ATP carrier CAT can be used as antigen in the study of anti-the carrier antibody. |
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| Keywords: | beef heart mitochondria ADP/ATP carrier isolation |
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