甘肃裕固族9个STR基因座遗传多态性研究

目的　研究中国甘肃裕固族STR遗传结构。方法　选择 9个STR基因座 (D3S135 8,VWA ,FGA ,TH0 1,TPOX ,CSF1PO ,D5S818,D13S317,D7S82 0 ) ,采用STR复合扩增及荧光标记STR基因扫描技术 ,同时检测 12 0个裕固族健康无关个体血液样本。结果　 9个STR基因位点共检出 6 5个等位基因 ,基因频率分布在 0 .0 0 5 7～0 .5 795 ;基因型共有 178种 ,频率分布在 0 .0 114～ 0 .30 6 8之间 ;9个STR位点基因型分布均符合Hardy Weinberg平衡定律 (P >0 .0 5 ) ;9个STR位点多态信息量 (PIC)均大于 0 .6 0 5 4 ,杂合度 (H)均大于 0 .6 15 8,个体识别力 (DP)均大于 0 .82 2 6 ,非父排除率 (EPP)均大于 0 .5 0 17。结论　获得了中国甘肃裕固族 9个STR基因座的遗传多态性数据 ,丰富了中华民族基因数据库 ,在人类群体遗传学及法医学研究领域有重要应用价值。

Genetic polymorphisms of 9 STR loci in Yugu ethnic group in Gansu Province

Objective To study the STR genetic structure of Yugu ethnic group in Gansu Province. Methods Distribution for nine STR loci was determined in a Chinese Yugu ethnic group based on STR genescan marked by fluorescence. Results Sixty five alleles and 178 genotypes were observed in 120 unrelated Chinese Yugu individuals, with the corresponding gene frequency and genotype frequency being 0.0057-0.5795 and 0.0114 -0.3068, respectively. The genotypes of nine STR loci were in accordance with the Hardy Weinberg equilibrium ( P > 0.05 ). The statistical analysis of 9 STR loci showed PIC (polymorphism information content)≥0.6054, H (heterozygosity) ≥0.6158, DP (discrimination power) ≥0.8226, EPP(probabilities of paternity exclusion) ≥0.5017. Conclusion These results enrich the Chineses genetic database and play an important role in Chinese population genetic study and forensic medicine application.