雄黄对急性早幼粒细胞白血病细胞诱导凋亡和促进分化的双重作用

目的　了解雄黄治疗急性早幼粒细胞白血病 (APL)的机制。方法　以APL细胞株NB4细胞为体外模型 ,应用透射电镜观察细胞形态 ,MTT检测细胞生长增殖状态 ,流式细胞仪检测细胞凋亡 ,NBT还原试验反映细胞分化能力 ,并检测细胞表面分化抗原CD11b、CD3 3 表达的改变。结果　雄黄作用后 ,细胞同时出现凋亡和分化相关的形态改变 ;超微结构发现凋亡小体 ,同时部分细胞内出现分化颗粒 ;流式细胞仪检测线粒体膜表面抗原APO 2 .7表达上调 ;CD11b表达增加 ,CD3 3 表达减少 ,其调变程度弱于全反式维甲酸。结论　雄黄对急性早幼粒细胞白血病细胞同时具有诱导凋亡和促进部分分化的双重效应。

The dual effects of realgar on acute promyelocytic leukemia cell:inducing apoptosis and promoting differentiation

Objective To further understand the possible mechanism of realgar in the treatment of acute promyelocytic leukemia (APL). Methods APL cell line NB 4 was used as in vitro model. The changes of cell morphology were observed by transmission electron microscopy; cell growth and proliferation were detected by methyl thiazolyl tetrazolium (MTT) assay; cell apoptosis was determined by flow cytometry assay. Cell differentiation was reflected by Nitroblue tetrazolium (NBT) reduction ability and expression of cell membrane surface antigens CD 11b and CD 33 .Results Realgar treated NB 4 cells presented morphologically some features of apoptotic cells and differentiated cells, for apoptotic body and granules related with differentiation could be found by electron microscopy. The expression of APO2.7, which is located at the membrane of mitochondrial, was up regulated. The expression of CD 11b was increased while that of CD 33 was decreased with the modulation level inferior to that of ATRA. Conclusion Realgar has dual effects on APL cells: inducting apoptosis and promoting partial differentiation.