人胃肠癌组织中浸润淋巴细胞活性的原位分析

利用一组抗淋巴细胞亚群的单克隆抗体和免疫组化方法对63例进展期胃癌和肠癌组织中浸润单个核细胞,尤其是散在浸润淋巴细胞(主要是T淋巴细胞)的I_2(HLA—DR)抗原和OKT_9抗原的表达进行了分析。同时,无选择的选取了18例慢性胃炎和慢性肠炎活检标本做为对照。在癌肿标本中,散在浸润淋巴细胞的I_2抗原表达显著变异。少数标本中很难查见I_2~+T细胞,大部分病例只能查见少数细胞呈I_2~+。63例中仅有10例其I_2~+细胞占全部散在浸润淋巴细胞的10—30%,所有瘤组织中几乎无OKT_9~+浸润T细胞。这种表现完全不同于正常粘膜及炎症粘膜中的I_2~+浸润单个核细胞分布。这说明就大多数病例来说,肿瘤组织中浸润的T细胞多数是非活化的。

ANALYSIS IN SITU ON ACTIVITY OF TUMORINFILTRATING LYMPHOCYTES IN HUMAN STOMACH AND COLON CANCER

We have immunophenotyped the tumor-infiltrating mononuclear cells, especially the I_2 (HLA-DR) antigen and OKT_9 antigen expression on lymphocyte infiltrates to determine the activity of infiltrating T cells in 63 cases of advanced stomach and colon cancer. In addition, 18 specimens for biopsies from patients with chronic gastritis and colitis were used as controls. In most cancer specimens, the infiltrating T cells were defficient of I_2 antigen, only 10 out of 63 cases had about 10--30% I_2 positive infiltrating T cells, and nearly no OKT_9 positive infiltrating T lymphocytes. This finding is quite different from that in nomal mucosa and chronic inflammatory mucosa, with more I_2 positive lymphocytes in their infiltrates, indicating that the infiltrating T lymphocytes in tumor tissue inhibited by ome inhibitors present in their environment and contrabuting to the explanation why the tumor mmunity can not control the growth and spread of the tumor and lead to the elimination of the tumor.