尼古丁损伤大鼠肠系膜动脉内皮功能及卡托普利的干预作用

目的观察尼古丁对大鼠离体肠系膜动脉内皮依赖性舒张反应的影响以及卡托普利的干预作用。方法应用器官培养技术和离体血管环张力描记法,研究不同浓度的尼古丁(10-5、10-4、10-3mol/L)在有或无卡托普利(0.01、0.030、.1 mmol/L)的情况下,对培养24 h的成年SD大鼠肠系膜动脉环(1 mm)内皮依赖性舒张反应的影响。结果尼古丁呈浓度依赖性损伤乙酰胆碱(ACh)诱导的内皮依赖性血管舒张反应(P<0.01,与空白对照组比较)。卡托普利呈浓度依赖性改善尼古丁对血管内皮依赖性舒张反应的损害。低浓度时对尼古丁损伤没有明显影响,高浓度具有显著的保护作用(P<0.05,与尼古丁10-4mol/L组比较)。结论卡托普利对尼古丁所引起的血管内皮依赖性舒张功能的损伤具有明显的保护作用。

Protective effect of captopril on nicotine injury in rat mesenteric artery endothelium

Objective To observe the effect of nicotine on endothelium-dependent relaxation(EDR) and examine whether captopril may exert beneficial effects on nicotine-induced impairment of EDR in the isolated rat mesenteric arteries.Methods An organ culture system and artery ring tension recording method were used.The mesenteric artery rings(1mm) of adult Sprague-Dawley rats were cultured with different concentration of nicotine(10~(-5),10~(-4),10~(-3)mol/L) for 24 hours in presence or absence of different concentration captopril(0.01,0.03,0.1mmol/L).The cultured-artery rings were precontracted with noradrenalin(NE,1μmol/L) and subsequently relaxed by a commulative addition of ACh(10~(-9)-10~(-6)mol/L).Results Exposure to nicotine(10~(-5),10~(-4),10~(-3)mol/L) induced significant concentration-dependent inhibition of EDR response of isolated rat mesenteric artery rings to ACh(P<0.01,respectively).However,captopril attenuated the inhibition induced by nicotine in concentration-dependent manner.There was no significant difference between captopril(0.01mol/L) group and nicotine(10~(-4)mol/L) group(P>0.05).But the EDR of the high concentration groups were significantly potentiated compared to the arteries cultured only with nicotine(10~(-4)mol/L)(P<0.05).Conclusion Captopril can prevent the inhibition of endothelium-dependent relaxation of rat mesenteric arteries induced by nicotine in vitro.