青春期大鼠实验性精索静脉曲张模型的建立及其对睾丸的影响

目的　研究青春期精索静脉曲张对睾丸的损害 ,探讨精索静脉曲张不育的机理。方法　通过部分结扎左肾静脉建立青春期大鼠实验性精索静脉曲张 (ELV)模型 ,分别于术后 4周和 8周研究双侧睾丸的组织学改变并进行统计学分析。结果　成功建立了ELV模型。该模型大鼠的睾丸病理损害以生精上皮退化 ,精子发生阻滞 ,生精细胞脱落 ,曲精小管萎缩 ,间质水肿最为多见。损害为双侧性 ,且有累进性特点。统计学分析显示 ,ELV大鼠左右侧睾丸平均重量和曲精小管直径均较对照组显著减小 ,睾丸间质面积较对照组显著扩大。结论　ELV大鼠双侧睾丸有严重的累进性病理改变 ,这可能是精索静脉曲张不育的主要原因之一。该模型为研究青春期精索静脉曲张的病理生理及其对附睾的影响提供了有用工具

The creation of experimental varicocele model in adolescent rats and its effect on the testis

Objective To study the effect of experimental varicocele on the testis in adolescent rats and investigate the mechanism of the infertility resulting from varicocele. Methods A varicocele model was created by partial ligation of the left renal vein in adolescent rats. Four and eight weeks after creation of the vricocele, the histology of the testes was studied and the statistical analyses were made.Results An experimental varicocele model was successfully created by partially ligating the left renal vein in adolescent rats. The main pathological changes in the testis of the model rats were degeneration of germinal epithelium, tubular atrophy and interstitial edema, and these lesions were bilateral and progressive. The statistical analyses showed that the seights of the testis of the two sides were significantly decreased compared with those of the control group, the diameters of the seminiferous tubules were significantly reduced and the interstitial areas were augmented.Conclusion Experimental left varicocele during adolescence has severe bilateral and progressive damaging effects on the testes, which may be the most important reason for infertility resulting from varicocele during adulthood.