V(D)J重组起始阶段的研究进展与展望

重组激活基因(recombination activating gene)1和2编码的RAG1和RAG2蛋白通过对V(D)J重组起始阶段的调节作用,使得抗原受体基因重排严格地按组织、细胞发育阶段进行。本文将就RAG蛋白结合和催化断裂抗原受体基因机制作简要介绍。这些新发现明确了体内V(D)J重组发生的部位以及其出现错误时可能发生的地方,提示V(D)J重组机制不仅对淋巴细胞正常发育起关键性作用,而且对基因组不稳定性和淋巴系统恶性肿瘤的发生也具有重要意义。

Research advances and prospects of initiation of V(D)J recombination

The initiation of V(D)J recombination by the proteins encoded by the recombination activating genes RAG1 and RAG2 is carefully orchestrated to ensure that antigen receptor gene assembly occurs in the appropriate cell lineage and in the proper developmental order.Here we review the recent advances in the mechanisms of DNA binding and cleavage by the RAG proteins in antigen receptor genes.These advances suggest novel mechanisms for both the targeting and the mistargeting of V(D)J recombination,with implications for how the reaction contributes to genome instability and lymphoid malignancy.