| 川芎嗪对体外培养兔关节软骨细胞MMP-1、MMP-13和TIMP-1表达的影响 |
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| 引用本文： | 付婷,王锁良.川芎嗪对体外培养兔关节软骨细胞MMP-1、MMP-13和TIMP-1表达的影响[J].西安交通大学学报(医学版),2012,33(1):114-117. |
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| 作者姓名： | 付婷 王锁良 |
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| 作者单位： | 1. 西安交通大学生命科学与技术学院,陕西西安 710049;西安市红十字会医院中医骨科,陕西西安 710054
2. 西安交通大学医学院第一附属医院康复医学科,陕西西安,710061 |
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| 基金项目： | 陕西省科技计划项目(No.2008K16-06)~~ |
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| 摘 要： | 目的研究川芎嗪对IL-1β诱导的兔关节软骨细胞损伤的影响。方法体外分离培养兔关节软骨细胞,并以其为实验模型;将实验分为正常对照组、白介素组、川芎嗪+白介素组;MTT法检测细胞增殖情况;Real-time PCR检测MMP-1、MMP-13和TIMP-1的mRNA表达量。结果 MTT检测结果显示,川芎嗪+白介素组细胞增殖情况显著高于正常对照组和白介素组(P<0.05);Real-time PCR结果显示,川芎嗪+白介素组MMP-1和MMP-13的mRNA表达量显著低于白介素组(P<0.05),而TIMP-1的mRNA表达量则显著高于白介素组(P<0.05)。结论川芎嗪可抑制IL-1β对软骨细胞的炎症损伤,具有保护软骨细胞,延缓软骨基质降解的作用,临床上可作为OA的治疗用药。
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| 关 键 词： | 川芎嗪 软骨细胞 MMP-1 MMP-13 TIMP-1 |
Effects of ligustrazine on expressions of MMP-1,MMP-13 and TIMP-1 in rabbit articular chondrocytes cultured in vitro |
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| FU Ting
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WANG Suo-Liang.Effects of ligustrazine on expressions of MMP-1,MMP-13 and TIMP-1 in rabbit articular chondrocytes cultured in vitro[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(1):114-117. |
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| Authors: | FU Ting WANG Suo-Liang |
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| Institution: | 1.School of Life Science & Technology,Xi’an Jiaotong University,Xi’an 710049; 2.Department of Rehabilitation Medicine,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061;3.Department of Traditional Chinese Medical Orthopedics,Xi’an Red Cross Hospital,Xi’an 710054,China) |
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| Abstract: | Objective To investigate the effects of ligustrazine on interleukin-1β(IL-1β)-induced lesion of rabbit articular chondrocytes.Methods Rabbit articular chondrocytes cultured in vitro were randomly divided into normal control group,IL-1β group and ligustrazine + IL-1β group.Chondrocytes proliferation was detected by MTT assay.MMP-1,MMP-13 and TIMP-1 mRNA expressions were determined by real-time PCR technique.Results MTT assay showed that chondrocytes’ proliferation was significantly higher in ligustrazine + IL-1β group than in normal control group and IL-1β group(P<0.05).Real-time PCR analysis showed that MMP-1 and MMP-13 mRNA expressions in ligustrazine + IL-1β group were significantly lower than those in IL-1β group(P<0.05).Furthermore,TIMP-1 mRNA expression in ligustrazine + IL-1β group was higher than that in IL-1β group(P<0.05).Conclusion Ligustrazine may inhibit IL-1β’s inflammatory damage on chondrocytes,thus protecting chondrocytes and delaying cartilage matrix degradation.These results suggest that ligustrazine can act as an agent for osteoarthritis. |
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| Keywords: | ligustrazine chondrocyte MMP-1 MMP-13 TIMP-1 |
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