| miRNA-449家族靶向CCNE2对胃癌细胞生长的抑制调节作用 |
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| 引用本文： | 刘棣,黄辰,刁冬梅,汪建光,程遥,任磊鹏,党诚学.miRNA-449家族靶向CCNE2对胃癌细胞生长的抑制调节作用[J].西安交通大学学报(医学版),2012,33(5):544-548,559. |
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| 作者姓名： | 刘棣 黄辰 刁冬梅 汪建光 程遥 任磊鹏 党诚学 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院肿瘤外科,陕西西安,710061
2. 西安交通大学医学院遗传学与分子生物学系,环境与疾病相关基因教育部重点实验室,陕西西安710061 |
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| 基金项目： | 国家自然科学基金资助项目(No.30973489)~~ |
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| 摘 要： | 目的研究miRNA-449在胃癌细胞中对细胞增殖、凋亡的影响,以及对靶基因CCNE2(cyclin E2)表达的影响。方法荧光定量PCR检测人低分化胃癌细胞株BGC823瞬时转染miRNA-449a/b及抑制剂后的表达;流式细胞术分析转染后细胞凋亡变化。Western blot检测miRNA-449a/b对靶基因表达的影响。结果在转染后,miRNA-449a/b组在48、72h对肿瘤细胞的增殖有明显的抑制作用(P<0.05),miRNA-449a/b组对肿瘤细胞凋亡有明显的促进作用(P<0.05),对CCNE2蛋白表达有显著抑制作用(P<0.05)。结论 miRNA-449a/b可能通过CCNE2来发挥其抑制胃癌细胞生长的作用。
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| 关 键 词： | miRNA 胃癌细胞 细胞周期 凋亡 CCNE2(cyclin E2) |
miRNA-449 family inhibits gastric cancer cells by targeting CCNE2 |
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| LIU Di
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HUANG Chen
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DIAO Dong-mei
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WANG Jian-guang
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CHENG Yao
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REN Lei-peng
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DANG Cheng-xue.miRNA-449 family inhibits gastric cancer cells by targeting CCNE2[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(5):544-548,559. |
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| Authors: | LIU Di HUANG Chen DIAO Dong-mei WANG Jian-guang CHENG Yao REN Lei-peng DANG Cheng-xue |
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| Institution: | 1(1.Department of Oncology Surgery,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061,2.Department of Genetics and Molecular Biology, Medical School of Xi’an Jiaotong University/Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,Xi’an 710061,China) |
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| Abstract: | Objective To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2.Methods The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR.Flow cytometry was used to detect cell apoptosis rate.The target gene of miR-449a/b was detected by Western blot.Results At 48 and 72 h,miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis(P<0.05).The expression of CCNE2 was also inhibited significantly(P<0.05).Conclusion miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2. |
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| Keywords: | miRNA gastric cancer cell cell cycle apoptosis CCNE2(cyclin E2) |
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